Spectra™ Multicolor Low Range Protein Ladder
Spectra™ Multicolor Low Range Protein Ladder
Thermo Scientific™

Spectra™ Multicolor Low Range Protein Ladder

Thermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six proteins and synthetic peptides (1.7 to 40Read more
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Catalog NumberQuantity
26628250 μL
Catalog number 26628
Price (HKD)
1,637.00
Each
Add to cart
Quantity:
250 μL
Request bulk or custom format
Price (HKD)
1,637.00
Each
Add to cart
Thermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six proteins and synthetic peptides (1.7 to 40 kDa) for use as size standards for peptides and small proteins in gel electrophoresis and western blotting. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring of protein migration during SDS-PAGE
• Verifying western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and western blots
• Locating a protein of interest for excision from an unstained preparative gel

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodColorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
For Use With (Application)Tricine gels
Gel CompatibilityBolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol)40, 25, 15, 10, 4.6, 1.7 kDa
Quantity250 μL
Ready to LoadYes
Recommended ApplicationsTricine gels
Shipping ConditionApproved for shipment on Wet or Dry Ice
Number of Markers6
Product LineSpectra
Product TypeProtein Ladder
Size Range1.7 to 40 kDa
Stain TypeBlue, Orange, Green
System TypeWestern Blotting, SDS-PAGE
Unit SizeEach
Contents & Storage
Contents: one vial of 250 μL

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C

Frequently asked questions (FAQs)

Why do Thermo Scientific prestained protein ladders not show the real protein sizes?

Coupling of chromophores to proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. The apparent molecular weight of prestained protein standards is calibrated in the classical TRIS glycine-SDS Laemmli system, however prestained proteins may have different mobility in other electrophoresis buffer and gel systems. It should also be noted that the sizing of proteins by gel electrophoresis does not give an exact value and depends on the protein sequence and post-modification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The upper bands of the ladder are missing. What could be the reason?

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the proteins in Thermo Scientific protein ladders have a His-Tag or would otherwise react with an anti-His-Tag antibody?

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can Thermo Scientific protein ladders be detected by Strep-Tactin conjugates?

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why are the PageRuler and Spectra ladder bands detected with antibodies against eukaryotic proteins?

All PageRuler and Spectra ladder bands are recombinant prokaryotic proteins purified from E. coli cells. None of them are related to eukaryotic proteins, however this cannot exclude the possibility that the ladder proteins may possess an epitope that is cross-reactive with the antibody used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Spectra™ Multicolor Low Range Protein Ladder FAQs