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Citations & References (15)
Invitrogen™
Staphylococcus aureus (Wood strain without protein A) BioParticles™, fluorescein conjugate
The Molecular Probes™ BioParticles™ product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeastRead more
| Catalog Number | Quantity |
|---|---|
| S2851 | 10 mg |
Catalog number S2851
Price (HKD)
2,383.00
Each
Quantity:
10 mg
Price (HKD)
2,383.00
Each
The Molecular Probes™ BioParticles™ product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles™ products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry.
We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan (S. cerevisiae) BioParticles™ conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dye after conjugation). Unlike the fluorescence of fluorescein-labeled BioParticles™ conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor™, BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10.
BioParticles Specifications:
• Label (Ex/Em): Fluorescein (∼494/518 nm)
• Particle: S. aureus (Wood strain, without protein A)
• Opsonizing reagent available
Using BioParticles Products
BioParticles™ conjugates are provided as lyophilized powders. There are approximately 3 x 108 E. coli or S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid. BioParticles™ conjugates can be reconstituted in the buffer of your choice for use in phagocytosis assays. The fluorescence of BioParticles™ conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers. In addition to cellular applications, fluorescent BioParticles™ conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants. These small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways.
Find More BioParticles™ Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles™ products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes™ Handbook.
For pH-sensitive endocytosis assays, see our pHrodo™ BioParticles™ conjugates.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan (S. cerevisiae) BioParticles™ conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dye after conjugation). Unlike the fluorescence of fluorescein-labeled BioParticles™ conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor™, BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10.
BioParticles Specifications:
• Label (Ex/Em): Fluorescein (∼494/518 nm)
• Particle: S. aureus (Wood strain, without protein A)
• Opsonizing reagent available
Using BioParticles Products
BioParticles™ conjugates are provided as lyophilized powders. There are approximately 3 x 108 E. coli or S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid. BioParticles™ conjugates can be reconstituted in the buffer of your choice for use in phagocytosis assays. The fluorescence of BioParticles™ conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers. In addition to cellular applications, fluorescent BioParticles™ conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants. These small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways.
Find More BioParticles™ Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles™ products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes™ Handbook.
For pH-sensitive endocytosis assays, see our pHrodo™ BioParticles™ conjugates.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeFITC (Fluorescein)
FormLyophilized Powder
Quantity10 mg
Shipping ConditionRoom Temperature
SpeciesS. aureus
For Use With (Equipment)Fluorescence Microscope
Product LineBioParticles
Product TypeBioparticle Conjugate
pH3 to 10
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Frequently asked questions (FAQs)
Are the Invitrogen BioParticles products sterile?
What is the type of bond that attaches the dyes to the BioParticles probes?
Citations & References (15)
Citations & References
Abstract
Phagocytosis and peroxidase release by seabream (Sparus aurata L.) leucocytes in response to yeast cells.
Journal:Anat Rec A Discov Mol Cell Evol Biol
PubMed ID:12704699
A flow cytometric method was adapted to evaluate phagocytosis by gilthead seabream leucocytes after incubation with yeast cells (Saccharomyces cerevisiae). Head-kidney leucocytes were incubated in vitro for different times in different proportions with heat-killed fluorescein isothiocyanate (FITC)-labeled yeast cells to study the kinetics of phagocytosis. Attached and internalized yeast cells
Conserved role of a complement-like protein in phagocytosis revealed by dsRNA knockout in cultured cells of the mosquito, Anopheles gambiae.
Journal:Cell
PubMed ID:11257225
'We characterize a novel hemocyte-specific acute phase glycoprotein from the malaria vector, Anopheles gambiae. It shows substantial structural and functional similarities, including the highly conserved thioester motif, to both a central component of mammalian complement system, factor C3, and to a pan-protease inhibitor, alpha2-macroglobulin. Most importantly, this protein serves as
Simultaneous flow cytometric method to measure phagocytosis and oxidative products by neutrophils.
Journal:Cytometry
PubMed ID:1782835
'We developed a rapid and sensitive two-color flow cytometric method which allows the simultaneous quantification of both the phagocytosis rate and the oxidative burst activation of polymorphonuclear leukocytes (PMNLs). The oxidation of hydroethidine (HE) to ethidium bromide (EB) was performed by the oxidative neutrophil products within the cells during the
Increase in phagocytosis after geldanamycin treatment or heat shock: role of heat shock proteins.
Journal:J Immunol
PubMed ID:16210633
'The response to injury is activated at the systemic and cellular levels. At the systemic level, phagocytosis plays a key role in controlling infections and clearing necrotic and apoptotic cells. The expression of heat shock proteins (Hsp), which is a well-conserved process, is a major component of cellular response to
Quantitation of phagocytosis by confocal microscopy.
Journal:Methods Enzymol
PubMed ID:10506981
'Confocal microscopy is an excellent tool to quantify phagocytosis. Depending on the particle used, phagocytosis can be determined by the simple manual counting of internalized particles. If a fluorescence probe is utilized, an analysis of fluorescence intensity can be used for quantification. The basic procedure can be altered in a