RNaseOUT™ Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used to avoid RNA degradation in a variety of applications. RNaseOUT™ Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of ∼52 kDa. RNaseOUT™ inhibits RNase A, RNase B, and RNase C.

Applications
cDNA synthesis, RT-PCR, and in vitro transcription and translation

Source
Purified by affinity chromatography from E. coli expressing a cloned porcine gene

Performance and quality testing
SDS-PAGE purity, endodeoxyribonuclease assay, protein concentration, specific activity, performance evaluated by RT-PCR

Unit definition
One unit inhibits 5 ng of RNase A by 50% using cytidine 2´, 3´ cyclic monophosphate (cCMP) as the substrate

Unit reaction conditions
100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A in 1 mL from 0 to 10 min at 25°C