Trypsin-EDTA (0.25%), phenol red
Trypsin-EDTA (0.25%), phenol red
引用資料與參考文獻 (2)
Gibco™

Trypsin-EDTA (0.25%), phenol red

This liquid formulation of trypsin contains EDTA and phenol red. Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture深入閱讀
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產品號碼Quantity
25200056100 mL
25200072500 mL
2520011420 x 100 mL
產品號碼 25200056
價格 (HKD)
167.00
Each
新增至購物車
Quantity:
100 mL
Customize this product
價格 (HKD)
167.00
Each
新增至購物車
This liquid formulation of trypsin contains EDTA and phenol red. Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco trypsin solutions and recommended dissociation conditions.

This trypsin solution is modified as follows:
With
• EDTA
• Phenol Red

The complete formulation is available.

We offer a variety of trypsin formulations, including animal origin-free TrypLE reagents.

Quality testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots are tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented traceability
We can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco Trypsin-EDTA (0.25%), phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
規格
Cell TypeMammalian Cells
ChelatorsEDTA
Concentration1X
Osmolality270 - 320 mOsm/kg
Product LineGibco
Quantity100 mL
Shelf Life24 Months
Shipping ConditionWet Ice
SourceAnimal Origin
Tested ForIn vitro Bioassay
ClassificationAnimal Origin
FormLiquid
Product TypeCell Culture Dissociation Reagent
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesPhenol Red, EDTA
pH7.2 to 8
Unit SizeEach
內容物與存放
Storage conditions: -5°C to -20°C
Shipping conditions: Wet ice
Shelf life: 24 months from date of manufacture

常見問答集 (常見問題)

What are the storage conditions for the Trypsin-EDTA (0.25%), phenol red (Cat. No. 25200072)?

We recommend storing the Trypsin-EDTA (0.25%), phenol red (Cat. No. 25200072) at  -5°C to -20°C as specified in the Contents & Storage section of the Thermofisher Scientific product page for this product. Although it is possible to store the trypsin for up to one week at +4°C, we would recommend preparing aliquots and storing them at -5°C to -20°C. These aliquots should be used up after thawing and freeze/thaw cycles should be avoided.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep trypsin in the refrigerator after thawing?

Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

What are the benefits of Gibco TrypLE reagent over trypsin?

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

View all Trypsin-EDTA (0.25%), phenol red, 100 mL FAQs

引用資料與參考文獻 (2)

引用資料與參考文獻
Abstract
Electrophoretic profiling of both RNA and protein from a single 250-pL sample.
Authors: Zabzdyr Jennifer L; Lillard Sheri J;
Journal:Anal Chem
PubMed ID:11985318
'A novel approach is described that uses capillary electrophoresis (CE) to electrophoretically sample and separate both protein and RNA from a single injected plug of cell lysate. A 250-pL sample of lysate from Chinese hamster ovary cells (9.6 x 10(7) cells/mL) was hydrodynamically injected into a capillary containing a Tris-based ... More
Characterization of the 46-kDa intermediates of matrix metalloproteinase 3 (stromelysin 1) obtained by site-directed mutation of phenylalanine 83.
Authors: Benbow U; Butticè G; Nagase H; Kurkinen M;
Journal:J Biol Chem
PubMed ID:8631880
The precursor of matrix metalloproteinase 3 (MMP-3/ stromelysin 1) is activated in vitro by proteinases or mercurial compounds by stepwise processes which include the initial formation of short-lived intermediates and the subsequent intermolecular cleavage of the His82-Phe83 bond to generate the fully activated mature MMP-3 (Nagase, H., Enghild, J. J., ... More