MagMAX™ HMW DNA Kit and Booster

The MagMAX HMW DNA Kit is a magnetic bead-based nucleic acid purification kit for isolation of high-quality, high molecular weight (HMW) DNA from whole blood, cells, and tissue. This versatile kit is optimized for both automated workflows with KingFisher purification systems and manual isolation processes, providing flexibility and scalability. It is specifically designed to obtain intact DNA fragments larger than 100 kb, making it excellent for long-read sequencing and various downstream molecular applications.

The MagMAX HMW DNA Kit is for use with fresh and frozen whole blood, while the MagMAX HMW DNA Kit with Cell and Tissue Extraction Buffer is also compatible with cell and tissue sample types. Already have MagMAX DNA Multi-Sample Ultra 2.0 and/or MagMAX Cell and Tissue Extraction Buffer? No problem, we offer MagMAX HMW DNA Booster separately in the 100 prep size found in the kit or a 1000 prep size for higher throughput.

Features of the MagMAX HMW DNA Kit include:

• Reproducibility and efficiency—consistently yields intact DNA fragments exceeding 100 kb from a range of sample types
• Versatile solution—flexible workflows for low- to high-throughput sample processing with manual or automated protocols
• Fast and optimized workflow—eliminates the need for post-processing steps or overnight elution
• Innovate your research—optimal performance with long-read systems such as Oxford Nanopore Technologies and PacBio systems
• Automation compatibility
• Available as stand-alone products—reagents available in larger volume bottles sold separately to meet your scalability needs

Versatility for downstream applications

Start your workflow out right with the MagMAX HMW DNA Kit, designed to meet the high demand for high-quality nucleic acid isolation, a vital component for highly sensitive downstream applications. The MagMAX HMW DNA Kit has optimized workflows available with the KingFisher purification systems, enabling a semi-automated workflow with a total turnaround time of less than two hours. This versatile kit is also optimized for lower throughput manual workflows, making it an excellent choice for laboratories of all sizes and throughput needs. Experience the perfect blend of efficiency, flexibility, and reliability with the MagMAX HMW DNA Kit for your downstream molecular applications, such as long-read sequencing.

Isolate HMW DNA from multiple sample types with a single sample prep kit

The MagMAX HMW DNA Kit leverages the innovative MagMAX HMW DNA Booster to enhance the elongation of fragment size. It is designed to isolate large fragment gDNA (100–300 kb) from various sample types, including 200 μL of fresh or frozen whole blood, one million cultured cells, and 2–8 mg tissue samples. Dynabeads magnetic beads with optimized surface chemistry and high specific surface area enable efficient kinetics and high sensitivity in capturing nucleic acids. The robust chemistry and workflow design minimizes impurities, such as proteins and detergents, that can interfere with downstream molecular analysis. Finally, the HMW genomic DNA is released from the beads using a low quantity TE elution solution.

The MagMAX HMW DNA Kit leverages the MagMAX DNA Multi-sample Ultra 2.0 Kit chemistry. This kit is specifically designed for the extraction of high molecular weight DNA from fresh and frozen whole blood samples. For cell and tissue samples, the MagMAX HMW DNA Kit with Cell and Tissue DNA Extraction Buffer provides an optimized solution, delivering reliable and efficient nucleic acid isolation. Existing users of the MagMAX DNA Multi-sample Ultra 2.0 Kit (A36570) can enhance their workflow by incorporating the MagMAX HMW DNA Booster (A36624, A36625) for whole blood, along with the MagMAX Cell and Tissue DNA Extraction Buffer (A45469, A45470) for cells and tissue. This combination enables the isolation of longer DNA fragments, thus broadening the scope of your genomic research.