SilverQuest™ Silver Staining Kit

引用資料與參考文獻 (5)

Invitrogen™

SilverQuest™ Silver Staining Kit

The SilverQuest Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels.深入閱讀

產品號碼	Quantity
LC6070	1 kit

產品號碼 LC6070

價格 (HKD)

3,163.00

新增至購物車

Quantity:

1 kit

Request bulk or custom format

價格 (HKD)

3,163.00

新增至購物車

The SilverQuest Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels. It is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis.

Compare all silver stains ›

Features of this kit:
• Compatible—staining compatible with mass spectrometry analysis
• Protocol—protein bands (0.3 ng of protein) can be visualized within an hour or for added speed a microwave procedure can be completed in ∼30 minutes
• Color-coded solutions—color-coded solutions for easy staining protocol

Mass spectrometry-compatible reagents
The SilverQuest Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so it leaves lysine and arginine side-chains unmodified. Additionally, the SilverQuest destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion and gel extraction and yield better coverage during mass spectrometry analysis.

For Research Use Only. Not for use in diagnostic procedures.

Detection LocationIn-Gel Detection

Detection MethodColorimetric

Product LineSilverQuest

Product TypeProtein Gel Staining Kit

Quantity1 kit

Shelf Life6 Months

Target MoleculeProtein

Label or DyeSilverQuest Silver Stain

Unit SizeEach

內容物與存放

This kit contains sufficient reagents to stain 25 minigels

Contains:
250 mL Sensitizer
25 mL Stainer
250 mL Developer
2 mL Developer Enhancer
250 mL Stopper solution
60 mL Destainer A
60 mL Destainer B

Store kit at room temperature (15°C to 30°C). Stable for 6 months.

常見問答集 (常見問題)

How much protein is required for mass spectrometry analysis by MALDI-TOF?

In general, the required amount needed to identify a gel-separated protein by enzymatic digestion (usually Trypsin) is about 2 pmole (equivalent to approximately 0.12 µg). Our SilverQuest Silver staining kit is mass spectrometry compatible.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

If you need to stop in the middle of the SilverQuest or SilverXpress silver staining procedure, at what point should this be done?

The sensitizing step is the optimal point to stop the procedure. The gel can be left overnight in the Sensitizing Solution. Although some other kits recommend leaving gels in the fix step, we have found that overnight fixation diminishes stain performances.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of Coomassie staining and silver staining?

Coomassie sensitivity: 50-500 ng protein per band
Silver-staining sensitivity: 1-5 ng protein per band
In general, silver staining is 10-100 times more sensitive than Coomassie staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverQuest Silver Staining kit and I see negative staining (a dark halo enclosing a pale area). Can you please help me troubleshoot?

This is usually due to overloading of the protein sample. We recommend decreasing the protein load per band. For an unknown protein, a serial dilution may be necessary to determine the best amount to load for a particular protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverQuest Silver Staining kit and it took a longer time for band development resulting in a dark background. What happened?

Here are possible causes and solutions:

- Low protein load. Increase the amount of protein load. Be sure to have at least 1-5 ng protein on the gel.
- Some proteins may need longer fixing time. Increase the time for fixing the gel to 2 hours or overnight.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all SilverQuest™ Silver Staining Kit FAQs

引用資料與參考文獻 (5)

引用資料與參考文獻

Abstract

The Wnt signaling inhibitor dickkopf-1 is required for reentry into the cell cycle of human adult stem cells from bone marrow.

Authors:Gregory CA, Singh H, Perry AS, Prockop DJ,

Journal:J Biol Chem

PubMed ID:12740383

'Adult human mesenchymal stem cells from bone marrow stroma (hMSCs) differentiate into numerous mesenchymal tissue lineages and are attractive candidates for cell and gene therapy. When early passage hMSCs are plated or replated at low density, the cultures display a lag phase of 3-5 days, a phase of rapid exponential ... More

Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release.

Authors:Takao-Rikitsu E, Mochida S, Inoue E, Deguchi-Tawarada M, Inoue M, Ohtsuka T, Takai Y,

Journal:J Cell Biol

PubMed ID:14734538

'We have recently isolated a novel cytomatrix at the active zone (CAZ)-associated protein, CAST, and found it directly binds another CAZ protein RIM1 and indirectly binds Munc13-1 through RIM1; RIM1 and Munc13-1 directly bind to each other and are implicated in priming of synaptic vesicles. Here, we show that all ... More

The R116C Mutation in alpha A-crystallin Diminishes Its Protective Ability against Stress-induced Lens Epithelial Cell Apoptosis.

Authors: Andley Usha P; Patel Harendra C; Xi Jing-Hua;

Journal:J Biol Chem

PubMed ID:11756414

'alphaA-crystallin is a small heat-shock protein expressed preferentially in the lens and is detected during the early stages of lens development. Recent work indicates that the expression of alphaA-crystallin enhances lens epithelial cell growth and resistance to stress conditions. Mutation of the arginine 116 residue to cysteine (R116C) in alphaA-crystallin ... More

The formation of highly soluble oligomers of alpha-synuclein is regulated by fatty acids and enhanced in Parkinson's disease.

Authors:Sharon R, Bar-Joseph I, Frosch MP, Walsh DM, Hamilton JA, Selkoe DJ,

Journal:Neuron

PubMed ID:12597857

Accumulation of misfolded proteins as insoluble aggregates occurs in several neurodegenerative diseases. In Parkinson's disease (PD) and dementia with Lewy bodies (DLB), alpha-synuclein (alpha S) accumulates in insoluble inclusions. To identify soluble alpha S oligomers that precede insoluble aggregates, we probed the cytosols of mesencephalic neuronal (MES) cells, normal and ... More

The semaphorin receptor plexin-B1 signals through a direct interaction with the Rho-specific nucleotide exchange factor, LARG.

Authors: Aurandt Jennifer; Vikis Haris G; Gutkind J Silvio; Ahn Natalie; Guan Kun-Liang;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12196628

Semaphorins are axon guidance molecules that signal through the plexin family of receptors. Semaphorins also play a role in other processes such as immune regulation and tumorigenesis. However, the molecular signaling mechanisms downstream of plexin receptors have not been elucidated. Semaphorin 4D is the ligand for the plexin-B1 receptor and ... More