NuPAGE™ Sample Reducing Agent (10X)
NuPAGE™ Sample Reducing Agent (10X)
引用資料與參考文獻 (2)
Invitrogen™

NuPAGE™ Sample Reducing Agent (10X)

NuPAGE Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains 500 mM dithiothreitol深入閱讀
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產品號碼Quantity
NP0004250μL
NP000910mL
產品號碼 NP0004
價格 (HKD)
160.00
Each
新增至購物車
Quantity:
250μL
價格 (HKD)
160.00
Each
新增至購物車
NuPAGE Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains 500 mM dithiothreitol (DTT) for a 10X concentration in a stabilized liquid form.

See all available buffers and reagents available for SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.
規格
Concentration500 mM
Product LineNuPAGE
Product TypeSample Reducing Agent
Quantity250μL
Shelf Life6 Months
Shipping ConditionWet Ice
Unit SizeEach
內容物與存放
NuPAGE™ Sample Reducing Agent (500 mM dithiothreitol) should be stored at 4°C. Guaranteed stable for 6 months unless otherwise specified in the product literature.

常見問答集 (常見問題)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Where do I find buffer recipes for your precast protein gels?

The formulations of buffers for our precast protein gels can be found at this link: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-electrophoresis-buffers-reagents.html

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ Sample Reducing Agent (10X), 250μL FAQs

引用資料與參考文獻 (2)

引用資料與參考文獻
Abstract
Apolipoprotein A-I is a selective target for myeloperoxidase-catalyzed oxidation and functional impairment in subjects with cardiovascular disease.
Authors:Zheng L, Nukuna B, Brennan ML, Sun M, Goormastic M, Settle M, Schmitt D, Fu X, Thomson L, Fox PL, Ischiropoulos H, Smith JD, Kinter M, Hazen SL,
Journal:J Clin Invest
PubMed ID:15314690
In recent studies we demonstrated that systemic levels of protein-bound nitrotyrosine (NO(2)Tyr) and myeloperoxidase (MPO), a protein that catalyzes generation of nitrating oxidants, serve as independent predictors of atherosclerotic risk, burden, and incident cardiac events. We now show both that apolipoprotein A-I (apoA-I), the primary protein constituent of HDL, is ... More
The myostatin propeptide and the follistatin-related gene are inhibitory binding proteins of myostatin in normal serum.
Authors: Hill Jennifer J; Davies Monique V; Pearson Adele A; Wang Jack H; Hewick Rodney M; Wolfman Neil M; Qiu Yongchang;
Journal:J Biol Chem
PubMed ID:12194980
Myostatin, also known as growth and differentiation factor 8, is a member of the transforming growth factor beta superfamily that negatively regulates skeletal muscle mass (1). Recent experiments have shown that myostatin activity is detected in serum by a reporter gene assay only after activation by acid, suggesting that native ... More