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引用資料與參考文獻 (17)
Invitrogen™
PowerLoad™ Concentrate, 100X
PowerLoad™ is an optimized formulation of nonionic, Pluronic™ surfactant polyols for the solubilization of water-insoluble dyes and other materials in深入閱讀
| 產品號碼 | Quantity |
|---|---|
| P10020 | 5 mL |
產品號碼 P10020
價格 (HKD)
1,531.00
Each
Quantity:
5 mL
價格 (HKD)
1,531.00
Each
PowerLoad™ is an optimized formulation of nonionic, Pluronic™ surfactant polyols for the solubilization of water-insoluble dyes and other materials in physiological media. These surfactants, for instance Pluronic™ F-127, have been used to help disperse acetoxymethyl (AM) esters of fluorescent ion indicators such as fluo-4, fura-2, indo-1, fluo-3, and SBFI; they appear to be required for loading of other dyes (e.g. SBFI-AM or PBFI-AM). The use of PowerLoad™ is optional with red shifted calcium indicators and other large molecular weight AM ester dyes, and may also be useful for dispersing other lipophilic probes. The concentration of Pluronic™ surfactants in PowerLoad™ is less than 0.2%. PowerLoad™ is effective in combination with water soluble Probenecid (P36400) to aid AM ester dye-loading and retention in cells that actively extrude the de-acetylated form through anion pumps. Together, these reagents allow for maximal loading of dyes with a minimum of effort in both imaging and high throughput screening (HTS) applications. Appropriate controls should be performed to make certain that PowerLoad™ is not altering the membrane properties of the cell.
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
For Research Use Only. Not for use in diagnostic procedures.
規格
Recommended StorageStore in refrigerator (2–8°C).
Quantity5 mL
Unit SizeEach
常見問答集 (常見問題)
Can the Fluo-4 Calcium Imaging Kit (Cat. No. F10489) components be purchased separately?
引用資料與參考文獻 (17)
引用資料與參考文獻
Abstract
The Ste20 kinases Ste20-related proline-alanine-rich kinase and oxidative-stress response 1 regulate NKCC1 function in sensory neurons.
Journal:J Biol Chem
PubMed ID:19307180
'NKCC1 is highly expressed in dorsal root ganglion neurons, where it is involved in gating sensory information. In a recent study, it was shown that peripheral nerve injury results in increased NKCC1 activity, not due to an increase in cotransporter expression, but to increased phosphorylation of the cotransporter (Pieraut, S.,
A novel initiation mechanism of death in Streptococcus pneumoniae induced by the human milk protein-lipid complex HAMLET and activated during physiological death.
Journal:J Biol Chem
PubMed ID:22700972
'To cause colonization or infection, most bacteria grow in biofilms where differentiation and death of subpopulations is critical for optimal survival of the whole population. However, little is known about initiation of bacterial death under physiological conditions. Membrane depolarization has been suggested, but never shown to be involved, due to
Osteoclast response to low extracellular sodium and the mechanism of hyponatremia-induced bone loss.
Journal:J Biol Chem
PubMed ID:21135109
'Our recent animal and human studies revealed that chronic hyponatremia is a previously unrecognized cause of osteoporosis that is associated with increased osteoclast numbers in a rat model of the human disease of the syndrome of inappropriate antidiuretic hormone secretion (SIADH). We used cellular and molecular approaches to demonstrate that
Receptor-selective diffusion barrier enhances sensitivity of astrocytic processes to metabotropic glutamate receptor stimulation.
Journal:Sci Signal
PubMed ID:22472649
Metabotropic glutamate receptor (mGluR)-dependent calcium ion (Ca²+) signaling in astrocytic processes regulates synaptic transmission and local blood flow essential for brain function. However, because of difficulties in imaging astrocytic processes, the subcellular spatial organization of mGluR-dependent Ca²+ signaling is not well characterized and its regulatory mechanism remains unclear. Using genetically
Transient Ca2+ mobilization caused by osmotic shock initiates salmonid fish sperm motility.
Journal:J Exp Biol
PubMed ID:22279070
Salmonid fish sperm motility is known to be suppressed in millimolar concentrations of extracellular K(+), and dilution of K(+) upon spawning triggers cAMP-dependent signaling for motility initiation. In a previous study, however, we demonstrated that suspending sperm in a 10% glycerol solution and subsequent dilution into a low-osmotic solution induced