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引用資料與參考文獻 (1)
Invitrogen™
Pacific Blue™ C5-Maleimide
Pacific Blue™ C5-maleimide is an excellent reagent for thiol-selective modification, quantitation and analysis. In this reaction, the thiol is added深入閱讀
| 產品號碼 | Quantity |
|---|---|
| P30506 | 1 mg |
產品號碼 P30506
價格 (HKD)
3,763.00
Each
Quantity:
1 mg
價格 (HKD)
3,763.00
Each
Pacific Blue™ C5-maleimide is an excellent reagent for thiol-selective modification, quantitation and analysis. In this reaction, the thiol is added across the double bond of the maleimide to yield a thioether. This compound does not react with methionine, histidine or tyrosine. Reaction of Pacific Blue™ C5-maleimide with amines usually requires a higher pH than reaction of maleimides with thiols.
View all Pacific Blue™ dye products..
View the Fluorophore Selection Guide.
View all Pacific Blue™ dye products..
View the Fluorophore Selection Guide.
For Research Use Only. Not for use in diagnostic procedures.
規格
Chemical ReactivityThiol
Detection MethodFluorescence
Emission455
Excitation410
Flow Cytometer Laser Lines405
FormatSolid
Label or DyePacific Blue™
Product TypeMaleimide
Quantity1 mg
Reactive MoietyMaleimide
Shipping ConditionRoom Temperature
SolubilityDMSO (Dimethylsulfoxide)
ColorOff-White
Label TypePacific Dyes
Product LineMolecular Probes, Pacific Blue
Unit SizeEach
內容物與存放
Contains 1 vial of Pacific Blue™ dye (1 mg). Store in freezer (-5 to -30°C) and protect from light.
引用資料與參考文獻 (1)
引用資料與參考文獻
Abstract
A robust method for production of MHC tetramers with small molecule fluorophores.
Journal:J Immunol Methods
PubMed ID:17187819
Tetramers of major histocompatibility complex molecules (MHC) are now well-established reagents for the detection of antigen-specific T cells by flow cytometry. MHC tetramers are prepared by mixing enzymatically biotinylated MHC molecules with commercial preparations of streptavidin, usually conjugated to a fluorescent phycobiliprotein such as phycoerythrin (PE) or allophycocyanin (APC). While