NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007
NuPAGE&trade; Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), <i>Contains 1 ea. LA0041, NP0004, NP0005, NP0007</i>
NuPAGE&trade; Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), <i>Contains 1 ea. LA0041, NP0004, NP0005, NP0007</i>
NuPAGE&trade; Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), <i>Contains 1 ea. LA0041, NP0004, NP0005, NP0007</i>
NuPAGE&trade; Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), <i>Contains 1 ea. LA0041, NP0004, NP0005, NP0007</i>
NuPAGE&trade; Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), <i>Contains 1 ea. LA0041, NP0004, NP0005, NP0007</i>
Invitrogen™

NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007

NuPAGE Tris-Acetate SDS Buffer Kit is designed for separation of medium to large size proteins on Tris-Acetate gels. This kitRead more
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Catalog NumberQuantity
LA00501 kit
Catalog number LA0050
Price (INR)
7,920.00
キャンペーン価格
Ends: 31-Dec-2025
14,393.00
Save 6,473.00 (45%)
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Quantity:
1 kit
Price (INR)
7,920.00
キャンペーン価格
Ends: 31-Dec-2025
14,393.00
Save 6,473.00 (45%)
Each
Add to cart
NuPAGE Tris-Acetate SDS Buffer Kit is designed for separation of medium to large size proteins on Tris-Acetate gels. This kit includes the following buffers:
• NuPAGE Tris-Acetate SDS Running Buffer (20X, Cat. No. LA0041)
• NuPAGE Sample Reducing Agent (10X, 250 μL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

See all available buffers and reagents available for SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Gel TypeSDS PAGE Gel
Quantity1 kit
Gel CompatibilityNuPAGE Tris-Acetate Gel
Product LineNuPAGE
Product TypeBuffer Kit
Unit SizeEach
Contents & Storage
NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels) contains:

• 20X NuPAGE™ Tris-Acetate SDS Running Buffer (500 ml): Store at 4°C to 25°C

• 10X NuPAGE™ Sample Reducing Agent (250 μl): Store at 2°C to 8°C

• NuPAGE™ Antioxidant (15 ml): Store at 4°C

• 4X NuPAGE™ LDS Sample Buffer (10 ml): Store at 4°C to 25°C

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can I use CTAB rather than SDS in my sample buffer?

No, CTAB will not work with any of our gels except for the NuPAGE Tris-Acetate gels. To use CTAB, you would need to use a running buffer of 50 mM acetic acid and 50 mM beta-alanine in equal concentrations. You would also need to switch the electrodes. Since CTAB is a cationic detergent, this would establish conditions for running a basic protein towards the anode (into the gel).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is there a reasonable replacement for the NuPAGE antioxidant for gel electrophoresis?

One can use 0.002% thioglycolic acid in the upper buffer reservoir. This is a good scavenger of free radicals. The reference to this is described by Hunkapiller et al, Methods of Enzymology, (91), 399, 1983. Caution should be taken when using this method since this compound is both toxic and expensive. In addition, the TGA must be fresh as it tends to become oxidized itself over time. Oxidized TGA will actually promote sample re-oxidation.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007 FAQs