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NeoBiotechnologies
EIF4A2 (Eukaryotic Initiation Factor 4A-II) Monoclonal Antibody (PCRP-EIF4A2-2B5)
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FIGURE: 1 / 2
EIF4A2 (Eukaryotic Initiation Factor 4A-II) Antibody (1974-MSM1-P0) in ARRAY
Analysis of Protein Array containing more than 19,000 full-length human proteins using EIF4A2 Mouse Monoclonal Antibody (PCRP-EIF4A2-2B5). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units... View More
Product Details
1974-MSM1-P0
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
PCRP-EIF4A2-2B5
Immunogen
Recombinant full-length human EIF4A2 protein
Conjugate
Form
Liquid
Concentration
200 µg/mL
Amount
20 µg
Purification
Protein A/G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Antibody is stable for 24 months.
Positive Control: HeLa cells. Cellular Localization: Nucleus.
Specificity Comments: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a5 cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.
Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish
Target Information
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amounts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP dependent RNA helicase eIF4A 2; ATP-dependent RNA helicase eIF4A-2; eIF-4A-II; Eukaryotic initiation factor 4A-II; eukaryotic translation initiation factor 4A isoform 2; N-terminally processed; unnamed protein product
Gene Aliases: BM-010; DDX2B; eIF-4A-II; EIF4A; eIF4A-II; EIF4A2; EIF4F; NEDHSS
UniProt ID: (Human) Q14240
Entrez Gene ID: (Human) 1974
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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