Nigericin, Free Acid
Nigericin, Free Acid
Citations & References (282)
Invitrogen™

Nigericin, Free Acid

Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K+/H+ ionophore nigericin, which causesRead more
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Catalog NumberQuantity
N149510 mg
Catalog number N1495
Price (KRW)
354,000
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Ends: 31-Mar-2026
404,000
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Quantity:
10 mg
Price (KRW)
354,000
Online offer
Ends: 31-Mar-2026
404,000
Save 50,000 (12%)
Each
Add to cart
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K+/H+ ionophore nigericin, which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K+.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
IndicatorIonophore
Molecular FormulaC40H68O11
Quantity10 mg
Recommended StorageStore in freezer (-5°C to -30°C)
Shipping ConditionRoom Temperature
Physical FormSolid
Product TypePotassium Ionophore
Unit SizeEach

Citations & References (282)

Citations & References
Abstract
Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy.
Authors:Martínez-Zaguilán R,Gurulé MW,Lynch RM
Journal:The American journal of physiology
PubMed ID:8967445
Measurement of intracellular pH.
Authors:Boyer MJ, Hedley DW
Journal:Methods Cell Biol
PubMed ID:7532259
CFTR regulates phagosome acidification in macrophages and alters bactericidal activity.
Authors:Di A, Brown ME, Deriy LV, Li C, Szeto FL, Chen Y, Huang P, Tong J, Naren AP, Bindokas V, Palfrey HC, Nelson DJ
Journal:Nat Cell Biol
PubMed ID:16921366
'Acidification of phagosomes has been proposed to have a key role in the microbicidal function of phagocytes. Here, we show that in alveolar macrophages the cystic fibrosis transmembrane conductance regulator Cl- channel (CFTR) participates in phagosomal pH control and has bacterial killing capacity. Alveolar macrophages from Cftr-/- mice retained the ... More
Rapid flow cytometric method for measuring senescence associated beta-galactosidase activity in human fibroblasts.
Authors:Noppe G, Dekker P, de Koning-Treurniet C, Blom J, van Heemst D, Dirks RW, Tanke HJ, Westendorp RG, Maier AB,
Journal:Cytometry A
PubMed ID:19777541
'Senescence associated-beta-galactosidase (SA-beta-gal) activity is a widely used marker for cellular senenescence. SA-beta-gal activity is routinely detected cytochemically, manually discriminating negative from positive cells. This method is time-consuming, subjective and therefore prone to operator-error. We aimed to optimize a flow cytometric method described by other workers using endothelial cells to ... More
Effects of cytoplasmic acidification on clathrin lattice morphology.
Authors:Heuser J
Journal:J Cell Biol
PubMed ID:2563729
'Reducing the internal pH of cultured cells by several different protocols that block endocytosis is found to alter the structure of clathrin lattices on the inside of the plasma membrane. Lattices curve inward until they become almost spherical yet remain stubbornly attached to the membrane. Also, the lattices bloom empty ... More
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