WesternBreeze™ Wash Solution (16X)
WesternBreeze™ Wash Solution (16X)
WesternBreeze™ Wash Solution (16X)
WesternBreeze™ Wash Solution (16X)
Invitrogen™

WesternBreeze™ Wash Solution (16X)

WesternBreeze® Wash Solution (16X) is an optimized solution of concentrated buffered saline containing detergent to minimize background and non-specific bindingRead more
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Catalog NumberQuantity
WB70032 x 100 mL
Catalog number WB7003
Price (KRW)
170,000
Online offer
Ends: 31-Mar-2026
199,000
Save 29,000 (15%)
2 x 100 mL
Add to cart
Quantity:
2 x 100 mL
Price (KRW)
170,000
Online offer
Ends: 31-Mar-2026
199,000
Save 29,000 (15%)
2 x 100 mL
Add to cart
WesternBreeze® Wash Solution (16X) is an optimized solution of concentrated buffered saline containing detergent to minimize background and non-specific binding on nitrocellulose (NC) and polyvinylidene difluoride (PVDF) membranes. Sufficient reagents for 20 mini-blots.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferBlotting Buffers
Quantity2 x 100 mL
Membrane CompatibilityNitrocellulose, PVDF
Product LineWesternBreeze
Product TypeWash Solution
Unit Size2 x 100 mL
Contents & Storage
2 x 100 ml Antibody Wash (16X)

Store at +4°C.

Frequently asked questions (FAQs)

Why is the actual band size on a western blot different from the predicted size of the protein?

Western blotting is based on the separation of proteins by their size on a gel. However, migration of proteins through the gel matrix is also affected by other factors, which may cause the observed band size to be different from the predicted size.

Common causes are:
-Post-translational modification; for example phosphorylation and glycosylation increase the size of the protein
-Post-translation cleavage; many proteins are synthesized as precursor proteins, and then cleaved to give the active form
-Multimers, for example dimerization of a protein. This is usually prevented under reducing conditions, although strong interactions can result in the appearance of higher bands
-Splice variants; alternative splicing may result in different sized proteins being produced from the same gene
-Relative charge; the composition of amino acids (charged vs. non-charged)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Can I purchase the Blocker/Diluent and Antibody Wash solutions from the WesternBreeze Chemiluminescent Detection kits as standalone products?

Yes, you may purchase them as standalone products using the Cat. Nos. listed below:

- Cat. No. WB7001 (Blocker/Diluent A)
- Cat. No. WB7002 (Blocker/Diluent B)
- Cat. No. WB7050 (Combo pack containing Blocker/Diluents A & B)
- Cat. No. WB7003 (Antibody Wash)

Note: Cat. Nos. WB7001 and WB7002 may be ordered by clicking the Quick Order button located at the top right of any page on our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I purchase the Blocker/Diluent and Antibody Wash solutions from the WesternBreeze Chromogenic Detection kits as standalone products?

Yes, you may purchase them as standalone products using the Cat. Nos. listed below:

- Cat. No. WB7003 (Antibody Wash)
- Cat. No. WB7001 (Blocker/Diluent A)
- Cat. No. WB7002 (Blocker/Diluent B)
- Cat. No. WB7050 (Combo pack containing Blocker/Diluents A & B)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.