50 bp DNA Ladder
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50 bp DNA Ladder
Invitrogen™

50 bp DNA Ladder

Green features
Invitrogen 50 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 50자세히 알아보기
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카탈로그 번호수량
1041601450 μg
카탈로그 번호 10416014
제품 가격(KRW)
247,000
Online offer
Ends: 31-Dec-2025
274,000
할인액 27,000 (10%)
Each
카트에 추가하기
수량:
50 μg
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
247,000
Online offer
Ends: 31-Dec-2025
274,000
할인액 27,000 (10%)
Each
카트에 추가하기
Invitrogen 50 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 50 bp to 2,500 bp. 50 bp DNA Ladder consists of 17 individual chromatography-purified DNA fragments and has reference bands at 2500, 800, and 350 bp for easy orientation.

50 bp DNA Ladder is ideal for separation on 2% agarose gels.

Highlights of 50 bp DNA Ladder:
Sharp, clear bands—chromatography purified fragments for consistent and reliable results
Convenient—provided with 6X TrackIt Cyan/Orange Loading Buffer for tracking of DNA migration
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 2% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

This ladder can be radiolabeled with T4 polynucleotide kinase or T4 DNA polymerase.

For Research Use Only. Not for use in diagnostic procedures.
사양
농도0.5 μg/μL
젤 호환성Agarose gel
그린 기능Sustainable packaging
반응 수100 Applications
제품 유형DNA Ladder
수량50 μg
로드 준비No
샘플 로딩 부피1 mL
배송 조건Approved for shipment at Room Temperature or on Dry Ice
기술Individual chromatography-purified DNA fragments
부피(미터법)100 μL
젤 유형Agarose
크기 범위50 to 2500 bp
Unit SizeEach
구성 및 보관
• 100 µL 50 bp DNA Ladder
• 1 mL 6X TrackIt Cyan/Orange Loading Buffer

Store at -20°C.

자주 묻는 질문(FAQ)

Can I know the sequences of Invitrogen DNA ladders?

Sequences of Invitrogen DNA and RNA ladders are proprietary.

Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?

Invitrogen DNA ladders contain linear dsDNA fragments.

Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?

Invitrogen DNA ladders are composed of double-stranded DNA fragments only.

I'm seeing anomalous migration of my DNA ladder. What happened?

This can happen if the marker was heated. Please ensure that the ladders are not heated before use.

What is the difference between the TrackIt DNA Ladders and other DNA ladders?

TrackIt DNA Ladders contain two tracking dyes in the sample buffers, which serve as visual markers for tracking electrophoresis progress through the gel, and also to indicate when maximum resolution is achieved. The tracking dyes should not obscure your visualization of DNA bands in the ladder, as the dyes run outside the limits of most DNA bands in the ladder.

TrackIt Cyan/Orange Loading Buffer is formulated with unique tracking dyes, Xylene Cyanol FF and Orange G. We recommend TrackIt Cyan/Orange Loading Buffer for DNA fragments between 10 bp and 1 kb.

The TrackIt Cyan/Yellow Loading Buffer is formulated with unique tracking dyes, Xylene Cyanol FF and Tartrazine. We recommend TrackIt Cyan/Yellow Loading Buffer for DNA fragments between 100 bp and 10 kb. The molecular weights are Xylene Cyanol FF, 638.6; Orange G, 452.4; Tartrazine, 534.4.

Note: The TrackIt DNA Ladders are not recommended for use with polyacrylamide gels and are not designed for quantitation.

인용 및 참조 문헌 (10)

인용 및 참조 문헌
Abstract
ChIP-Seq using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites.
Authors:Lefrançois P, Zheng W, Snyder M
Journal:Methods Enzymol
PubMed ID:20946807
'Much of eukaryotic gene regulation is mediated by binding of transcription factors near or within their target genes. Transcription factor binding sites (TFBS) are often identified globally using chromatin immunoprecipitation (ChIP) in which specific protein-DNA interactions are isolated using an antibody against the factor of interest. Coupling ChIP with high-throughput ... More
Post intrastromal corneal ring segments insertion complicated by Candida parapsilosis keratitis.
Authors:Mitchell BM, Kanellopoulos AJ, Font RL
Journal:Clin Ophthalmol
PubMed ID:23467516
'This case report describes the clinical and histopathologic features, including molecular confirmation, of fungal keratitis after intrastromal corneal ring segments placement for keratoconus. A 52-year-old woman underwent insertion of Intacs(®) corneal implants for treatment of keratoconus. Extrusion of the implants was noted 5 months post insertion and replaced. Three months ... More
Development of PCR-RFLP assay for the discrimination of Plasmodium species and variants of P. vivax (VK210, VK247 and P. vivax-like) in Anopheles mosquitoes.
Authors:Cassiano GC, Storti-Melo LM, Póvoa MM, Galardo AK, Rossit AR, Machado RL
Journal:Acta Trop
PubMed ID:21420375
'The identification of Plasmodium species in Anopheles mosquitoes is an integral component of malaria control programs. We developed a new assay to identify Plasmodium falciparum, Plasmodium malariae, and Plasmodium vivax variants. Specific primers were designed to hybridize to CS gene-specific regions. Polymerase chain reaction (PCR) and restriction fragment length polymorphism ... More
Report of an international collaborative study to evaluate the suitability of multiplex PCR as an identity assay for different sub-strains of BCG vaccine.
Authors:Markey K, Ho MM, Choudhury B, Seki M, Ju L, Castello-Branco LR, Gairola S, Zhao A, Shibayama K, Andre M, Corbel MJ
Journal:Vaccine
PubMed ID:20732463
'Current methods for the identification of BCG vaccine in quality control settings involve acid-fast staining with microscopic examination. However, this method is unable to distinguish the many different sub-strains of BCG, or to differentiate BCG strains from virulent members of the Mycobacterium tuberculosis complex. A multiplex PCR (mPCR) which uses ... More
Identification of novel mutation in cathepsin C gene causing Papillon-Lefèvre Syndrome in Mexican patients.
Authors:Romero-Quintana JG, Frías-Castro LO, Arámbula-Meraz E, Aguilar-Medina M, Dueñas-Arias JE, Melchor-Soto JD, Romero-Navarro JG, Ramos-Payán R
Journal:BMC Med Genet
PubMed ID:23311634
Papillon-Lefèvre Syndrome (PLS) is a type IV genodermatosis caused by mutations in cathepsin C (CTSC), with a worldwide prevalence of 1-4 cases per million in the general population. In México, the prevalence of this syndrome is unknown, and there are few case reports. The diagnosis of twenty patients in the ... More