DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples
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DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples
인용 및 참조 문헌 (10)
Invitrogen™

DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples

DNAzol™ Reagent는 동물, 식물, 효모, 세균 유래 고체 및 액체 샘플에서 유전체 DNA를 분리하는 완전한 ready-to-use 유기 시약입니다. DNAzol™ Reagent자세히 알아보기
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카탈로그 번호수량
10503027100 mL
카탈로그 번호 10503027
제품 가격(KRW)
328,000
Online offer
Ends: 31-Dec-2025
364,000
할인액 36,000 (10%)
Each
카트에 추가하기
수량:
100 mL
제품 가격(KRW)
328,000
Online offer
Ends: 31-Dec-2025
364,000
할인액 36,000 (10%)
Each
카트에 추가하기
DNAzol™ Reagent는 동물, 식물, 효모, 세균 유래 고체 및 액체 샘플에서 유전체 DNA를 분리하는 완전한 ready-to-use 유기 시약입니다. DNAzol™ Reagent 절차는 대개 10-30분 이내에 완료되며 DNA 수율은 70-100%입니다.

• 높은 활용성 – 방대한 범위의 샘플 물질에 사용할 수 있습니다.
• 높은 효율성– 유전체 DNA의 신속한 분리와 높은 수율

다양한 샘플 유형에서 효율적으로 유전체 DNA 분리
DNAzol™ Reagent 절차는 세포 용해물에서 DNA의 선택적 침전이 가능한 새로운 guanidine-detergent 용해액을 사용합니다. DNAzol™ Reagent 1 mg으로 전혈 0.1 ml 또는 조직 25-50 mg에서 DNA 1-3 x 107 cell을 분리할 수 있습니다.

유전체 DNA의 신속한 분리와 높은 회수율
DNAzol™ Reagent는 DNA 분리 프로토콜에 신뢰성, 효율성, 간편성을 모두 제공합니다. 분리 중 생물 샘플이 DNAzol™ Reagent에서 용해(또는 균질화)되어 유전체 DNA가 에탄올로 용해물에서 침전됩니다. 에탄올 세척 후 DNA를 물이나 8 mM NaOH에 용해시킬 수 있습니다. 전체 절차를 10-30분 내에 완료할 수 있으며 DNA 회수율은 70-100%입니다.

분리된 DNA는 추후 수 많은 어플리케이션에서 사용 가능합니다.
DNAzol™ 절차로 추출한 유전체 DNA는 Southern blotting, cloning, PCR, restriction endonuclease digestion, dot blot hybridization 등 여러 어플리케이션에 적합합니다.

이 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 사람에게 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용리량Varies
최종 제품 유형Genomic DNA
용도(애플리케이션)PCR, southern blotting, sequencing, nucleic acid labeling, hybridization
고처리량 호환성Not High-throughput Compatible (Manual)
수량100 mL
샘플 종류Bacteria, Cells, Plant, Tissue, Yeast
배송 조건Room Temperature
시작 물질 양Bacteria: ≤107 cells or 0.1 mL
Cells: ≤107
Plant: ≤50 mg
Tissue: ≤50 mg
Yeast: ≤107 cells or 0.1 mL
테스트 시간30 min.
Isolation TechnologyOrganic Extraction
Unit SizeEach
구성 및 보관
100 mL DNAzol Reagent; room temperature

자주 묻는 질문(FAQ)

I see two phases after addition of ethanol during DNA isolation using DNAzol Reagent. What should I do?

It is possible to see two phases after addition of ethanol if the amount of DNAzol Reagent was too low. Add more DNAzol Reagent and continue.

I've isolated my DNA using the DNAzol Reagent, but cannot cut it with restriction enzymes. What could be causing this?

If the cells or tissue were washed with phosphate buffer solutions prior to DNA isolation, the phosphate may have been carried over and be inhibiting restriction enzymes. We recommend adding DNAzol Reagent to the DNA solution and reprecipitating with 0.5 volumes of 95% EtOH. Wash twice with 95%, dry briefly, and resuspend in 8 mM NaOH.

The DNA I've isolated using the DNAzol Reagent is hard to resuspend. Do you have recommendations for fixing this?

You can try incubating samples resuspended in 8 mM NaOH at 37 degrees C overnight to resuspend the DNA. You can also try incubating at 45 degrees C for 15 minutes.

My DNAzol Reagent turned a dark murky green from its original light green. Is it still okay to use?

Yes, this happens due to the dye in the reagent, and seems to be dependent on the volume of the stored reagent. The color change does not affect its performance.

Will DNAzol Reagent isolate only genomic DNA or will plasmid DNA also be isolated? How about mitochondrial DNA?

The DNA isolated is actually total DNA, so plasmid DNA will be isolated along with genomic DNA. The mitochondrial genome is similar to a plasmid and can be isolated using DNAzol Reagent. The 1 minute room temperature incubation in ethanol before centrifugation should be extended to 5-10 minutes for maximum recovery.

View all DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples FAQs

인용 및 참조 문헌 (10)

인용 및 참조 문헌
Abstract
p21Cip-1/SDI-1/WAF-1 gene is involved in chondrogenic differentiation of ATDC5 cells in vitro.
Authors: Negishi Y; Ui N; Nakajima M; Kawashima K; Maruyama K; Takizawa T; Endo H;
Journal:J Biol Chem
PubMed ID:11406616
'Development of skeletal cartilage is characterized with coupling growth arrest and cell differentiation. Here, to understand the cyclin-dependent kinase inhibitors involved in the progression of chondrogenic differentiation, we examined changes in the expression levels of cyclin-dependent kinase inhibitor members using mouse ATDC5 prechondrocytes as a widely used in vitro model ... More
A new type of high affinity folic acid transporter in the protozoan parasite Leishmania and deletion of its gene in methotrexate-resistant cells.
Authors:Richard D, Kundig C, Ouellette M.
Journal:J Biol Chem
PubMed ID:12023977
'The protozoan parasite Leishmania is a folate auxotroph and thus depends on the uptake of folate from the environment to meet its folate requirement. We show here that Leishmania contains several putative pteridine transporter genes. Some of these genes are deleted in methotrexate-resistant Leishmania cells where there is no measurable ... More
Isolation of high-molecular-weight genomic DNA from intact biohazardous mammalian tissues.
Authors:Liska V; Ruprecht R M;
Journal:Biotechniques
PubMed ID:9894593
n/a
DNA extraction from fixed cytogenetic cell suspensions.
Authors:Amorim MR, Vargas FR, Llerena JC, Pombo-de-Oliveira MS
Journal:Genet Mol Res
PubMed ID:17985302
We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment ... More
Rapid and efficient extraction of genomic DNA from different phytopathogenic fungi using DNAzol reagent.
Authors:Guo JR, Schnieder F, Abd-Elsalam KA, Verreet JA
Journal:Biotechnol Lett
PubMed ID:15685411
A modified procedure using the commercial DNAzol reagent was successfully applied to extract genomic DNA from 25 fungal species. The DNA yield varied from 306 to 1,927 microg g(-1) dry mycelia and the A(260)/A(280) ratio from 1.59 to 1.93. Compared with the method of J.L. Cenis (Nucleic Acids Res. 1992, ... More
View all DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples citations