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인용 및 참조 문헌 (6)
Invitrogen™
RNaseOUT™ Recombinant Ribonuclease Inhibitor
RNaseOUT™ Recombinant Ribonuclease Inhibitor는 RNase A 같은 pancreatic-type ribonucleases을 억제하는 강력한 non-competitive inhibitor입니다. 다른 RNase inhibitor와 달리 RNaseOUT™ Recombinant Ribonuclease자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 10777019 | 5000 Units |
카탈로그 번호 10777019
제품 가격(KRW)
299,000
キャンペーン価格
Ends: 31-Dec-2025
332,000할인액 33,000 (10%)
Each
수량:
5000 Units
제품 가격(KRW)
299,000
キャンペーン価格
Ends: 31-Dec-2025
332,000할인액 33,000 (10%)
Each
RNaseOUT™ Recombinant Ribonuclease Inhibitor는 RNase A 같은 pancreatic-type ribonucleases을 억제하는 강력한 non-competitive inhibitor입니다. 다른 RNase inhibitor와 달리 RNaseOUT™ Recombinant Ribonuclease Inhibitor는 산성 단백질로 분자량은 ∼52 kDa입니다. RNaseOUT™는 RNase A, RNase B, RNase C를 억제합니다.
적용: cDNA 합성, RT-PCR (2), in vitro transcription 및 translation.
원천: cloned porcine gene을 발현하는 E. coli 에서 친화성 크로마토그래피로 정제.
성능 및 품질 검사: SDS-PAGE 순도, endodeoxyribonuclease assay, 단백질 농도, 특이 활성; 성능- RT-PCR로 평가.
단위 정의: 1 단위가 기질로 cytidine 2´, 3´ cCMP (cyclic monophosphate)를 사용해 RNase A 5 ng를 50% 억제합니다 (1).
단위 반응 조건: 100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A 1 ml: 0∼10분, 25°C.
적용: cDNA 합성, RT-PCR (2), in vitro transcription 및 translation.
원천: cloned porcine gene을 발현하는 E. coli 에서 친화성 크로마토그래피로 정제.
성능 및 품질 검사: SDS-PAGE 순도, endodeoxyribonuclease assay, 단백질 농도, 특이 활성; 성능- RT-PCR로 평가.
단위 정의: 1 단위가 기질로 cytidine 2´, 3´ cCMP (cyclic monophosphate)를 사용해 RNase A 5 ng를 50% 억제합니다 (1).
단위 반응 조건: 100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A 1 ml: 0∼10분, 25°C.
For Research Use Only. Not for use in diagnostic procedures.
사양
농도40 U/μL
제품라인RNaseOUT
수량5000 Units
Unit SizeEach
구성 및 보관
Store at -20°C. Avoid exposing product to frequent changes in temperature. RNaseOUT™ Ribonuclease Inhibitor requires 1 mM DTT to maintain activity.
The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation.
The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation.
자주 묻는 질문(FAQ)
Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?
What does RNaseOUT Recombinant RNase Inhibitor do?
What is the concentration of RNaseOUT Recombinant Ribonuclease Inhibitor in Units/microL?
What is the temperature limitation of RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)?
I am having trouble performing PCR amplification after DNA isolation with RNAlater-treated samples. Does RNAlater have an impact on downstream PCR applications?
인용 및 참조 문헌 (6)
인용 및 참조 문헌
Abstract
The catalytic domain of RNase E shows inherent 3' to 5' directionality in cleavage site selection.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12417756
'RNase E, a multifunctional endoribonuclease of Escherichia coli, attacks substrates at highly specific sites. By using synthetic oligoribonucleotides containing repeats of identical target sequences protected from cleavage by 2''-O-methylated nucleotide substitutions at specific positions, we investigated how RNase E identifies its cleavage sites. We found that the RNase E catalytic
Cloning and functional characterization of HDAC11, a novel member of the human histone deacetylase family.
Journal:J Biol Chem
PubMed ID:11948178
'We have cloned and characterized a human cDNA that belongs to the histone deacetylase family, which we designate as HDAC11. The predicted HDAC11 amino acid sequence reveals an open reading frame of 347 residues with a corresponding molecular mass of 39 kDa. Sequence analyses of the putative HDAC11 protein indicate
Development of reverse transcription (RT)-PCR and real-time RT-PCR assays for rapid detection and quantification of viable yeasts and molds contaminating yogurts and pasteurized food products.
Journal:Appl Environ Microbiol
PubMed ID:12839789
Reverse transcriptase PCR (RT-PCR) and real-time RT-PCR assays have been used to detect and quantify actin mRNA from yeasts and molds. Universal primers were designed based on the available fungal actin sequences, and by RT-PCR they amplified a specific 353-bp fragment from fungal species involved in food spoilage. From experiments
Regulation of H-ras splice variant expression by cross talk between the p53 and nonsense-mediated mRNA decay pathways.
Journal:Mol Cell Biol
PubMed ID:17709397
When cells are exposed to a genotoxic stress, a DNA surveillance pathway that involves p53 is activated, allowing DNA repair. Eukaryotic cells have also evolved a mechanism called mRNA surveillance that controls the quality of mRNAs. Indeed, mutant mRNAs carrying premature translation termination codons (PTCs) are selectively degraded by the
Biochemistry of mitochondrial nitric-oxide synthase.
Journal:J Biol Chem
PubMed ID:12154090
We reported that the generation of nitric oxide by mitochondria is catalyzed by a constitutive, mitochondrial nitric-oxide synthase (mtNOS). Given that this production may establish the basis for a novel regulatory pathway of energy metabolism, oxygen consumption, and oxygen free radical production, it becomes imperative to identify unequivocally and characterize