Advanced DMEM
Advanced DMEM
인용 및 참조 문헌 (2)
Gibco™

Advanced DMEM

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
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카탈로그 번호수량
1249102310 x 500 mL
12491015500 mL
카탈로그 번호 12491023
제품 가격(KRW)
475,000
Online offer
Ends: 31-Dec-2025
499,000
할인액 24,000 (5%)
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수량:
10 x 500 mL
Customize this product
제품 가격(KRW)
475,000
Online offer
Ends: 31-Dec-2025
499,000
할인액 24,000 (5%)
Each
카트에 추가하기

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.

This Advanced DMEM is manufactured as follows:

With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red

Without: L-glutamine

The complete formulation is available.

Using Advanced DMEM

Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use Only. Not for use in diagnostic procedures.
사양
세포주MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형DMEM (Dulbecco's Modified Eagle Medium)
수량10 x 500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin
형태Liquid
Serum LevelReduced Serum
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
첨가제 없음No Glutamine, No HEPES
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

What kind of ascorbic acid is present in Advanced DMEM (Cat. No. 12491015 or 12491023)?

Advanced DMEM contains 2.5 mg/L of ascorbic acid phosphate to maintain the stability. You do not need to supplement it with anything else.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Advanced DMEM FAQs

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
Surface functionalization of carbon nanomaterials by self-assembling hydrophobin proteins.
Authors:Yang W, Ren Q, Wu YN, Morris VK, Rey AA, Braet F, Kwan AH, Sunde M,
Journal:Biopolymers
PubMed ID:23097233
'Class I fungal hydrophobins are small surface-active proteins that self-assemble to form amphipathic monolayers composed of amyloid-like rodlets. The monolayers are extremely robust and can adsorb onto both hydrophobic and hydrophilic surfaces to reverse their wettability. This adherence is particularly strong for hydrophobic materials. In this report, we show that ... More
Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture.
Authors:Vacanti V, Kong E, Suzuki G, Sato K, Canty JM, Lee T,
Journal:J Cell Physiol
PubMed ID:15880640
'The in vitro culture of porcine bone marrow-derived mesenchymal stem cells (MSCs) was used for the investigation of adult stem cell biology. Isolated porcine MSCs possessed the ability to proliferate extensively in Gibco(R) Advanced DMEM containing 5% fetal bovine serum (FBS). Greater than 40 serial MSC passages and 100 cell ... More