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인용 및 참조 문헌 (276)
Invitrogen™

Lipofectin™ Transfection Reagent

Lipofectin® Transfection Reagent는 내피세포 transfection에 우수한 시약으로 일부 고객들은 특정 세포주에 DNA, RNA 또는 oligonucleotides을 transfection하는데도 사용합니다. Lipofectin®는 HeLa 세포자세히 알아보기
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카탈로그 번호수량
182920111 mL
182920374 mL
카탈로그 번호 18292011
제품 가격(KRW)
574,000
Online offer
Ends: 30-Jun-2026
655,000
할인액 81,000 (12%)
Each
수량:
1 mL
제품 가격(KRW)
574,000
Online offer
Ends: 30-Jun-2026
655,000
할인액 81,000 (12%)
Each
Lipofectin® Transfection Reagent는 내피세포 transfection에 우수한 시약으로 일부 고객들은 특정 세포주에 DNA, RNA 또는 oligonucleotides을 transfection하는데도 사용합니다. Lipofectin®는 HeLa 세포 transfection에서 PLUS® 시약과 병용하여 우수한 결과를 보였습니다(1).
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Transfection
고처리량 호환성Not High-throughput Compatible (Manual)
포함One vial (1mL) of Lipofectin Transfection Reagent
제품라인Lipofectin
제품 유형Transfection Reagent
수량1 mL
혈청 호환 가능No
배송 조건Wet Ice
셀 유형Established Cell Lines, Primary Cells, Hard-to-Transfect Cells
형식6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
샘플 종류Plasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
구성 및 보관
Contains one vial (1 ml) of Lipofectin™ Transfection Reagent. Store at 4°C. Do not freeze.

자주 묻는 질문(FAQ)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Do you offer a reagent for the transfection of endothelial cells?

We recommend using the Lipofectamine 3000 Reagent for transfection of endothelial cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

View all Lipofectin™ Transfection Reagent, 1 mL FAQs

인용 및 참조 문헌 (276)

인용 및 참조 문헌
Abstract
Characterization of human B creatine kinase gene regulation in the heart in vitro and in vivo.
Authors:Ritchie ME
Journal:The Journal of biological chemistry
PubMed ID:8810319
Isolation and characterization of a novel cDNA encoding a human UDP-glucuronosyltransferase active on C19 steroids.
Authors:Beaulieu M,Lévesque E,Hum DW,Bélanger A
Journal:The Journal of biological chemistry
PubMed ID:8798464
Transfection techniques for producing recombinant baculoviruses.
Authors:Trotter KM,Wood HA
Journal:Molecular biotechnology
PubMed ID:9067978
Cloning and functional expression of a thyrotropin receptor cDNA from rat fat cells.
Authors:Endo T; Ohta K; Haraguchi K; Onaya T;
Journal:J Biol Chem
PubMed ID:7738021
Thyrotropin receptor (TSH-R) has been thought to be thyroid-specific, but, by Northern blot analysis, we found that rat adipose tissue expressed TSH-R mRNAs in amounts approaching those in the thyroid. To investigate the function of TSH-R from adipose tissue, we screened a rat fat cell lambda gt11 cDNA library for ... More
Isolation and cDNA cloning of Ksp-cadherin, a novel kidney-specific member of the cadherin multigene family.
Authors:Thomson R B; Igarashi P; Biemesderfer D; Kim R; Abu-Alfa A; Soleimani M; Aronson P S;
Journal:J Biol Chem
PubMed ID:7615566
Cadherins are recognized as the principal mediators of homotypic cellular recognition and play a demonstrated role in the morphogenic direction of tissue development. We report here the identification of a structurally unique, kidney-specific member of the cadherin multigene family (Ksp-cadherin). cDNA cloning and molecular analysis of the 130-kDa protein confirmed ... More
View all Lipofectin™ Transfection Reagent, 1 mL citations