Neurobasal™ Medium
Neurobasal™ Medium
인용 및 참조 문헌 (5)
Gibco™

Neurobasal™ Medium

Neurobasal Medium은 Gibco B-27 Supplenment와 함께 사용할 때 astrocyte feeder layer가 필요 없으며, 순수한 태아(pre-natal) 및 배아 신경 세포 집단의자세히 알아보기
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카탈로그 번호수량
21103049500 mL
카탈로그 번호 21103049
제품 가격(KRW)
140,000
線上優惠
Ends: 31-Mar-2026
155,000
할인액 15,000 (10%)
Each
카트에 추가하기
수량:
500 mL
Customize this product
제품 가격(KRW)
140,000
線上優惠
Ends: 31-Mar-2026
155,000
할인액 15,000 (10%)
Each
카트에 추가하기
Neurobasal Medium은 Gibco B-27 Supplenment와 함께 사용할 때 astrocyte feeder layer가 필요 없으며, 순수한 태아(pre-natal) 및 배아 신경 세포 집단의 장기간 유지 및 성숙을 위해 설계된 기본 배지입니다.

Neurobasal 배지는 대부분의 신경 세포 응용 분야에서 사용하기에 적합합니다. 기타 다른 응용 분야를 위해 Neurobasal 배지 및 B-27 supplements의 대체 제형도 제공합니다.

Neurobasal Media를 사용하는 확립된 프로토콜을 확인하시려면 Gibco 신경생물학 프로토콜 핸드북을 참조하십시오.

Gibco CellCite에서 Gibco 신경생물학 제품을 사용한 논문을 확인하십시오.

연구용으로만 사용하십시오. 치료 또는 진단 목적으로 동물이나 사람에게 사용할 수 없습니다.
사양
세포 유형신경 세포
제품라인Neurobasal
제품 유형Neurobasal 배지
수량500 mL
유통 기한제조일로부터 12개월
배송 조건상온
테스트됨Endotoxin, 성능, 멸균성, 삼투압, pH
분류Serum-Free
Culture Type신경 세포 배양
형태액체
첨가제 포함Phenol Red
Unit SizeEach
구성 및 보관
차광하여 냉장 보관(2–8°C).

자주 묻는 질문(FAQ)

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does the Neurobasal Medium contain a higher concentration of L-cysteine, which is reported to activate NMDA receptors, according to the paper "Excitotoxicity Triggered by Neurobasal Culture Medium", http://dx.plos.org/10.1371/journal.pone.0025633?

The original published formulation of Neurobasal culture medium contained 10 µM L-cysteine. However, our Neurobasal media formulation contains 260 µM L-cysteine because it was shown to improve cell survival.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I maintain differentiated neurospheres in Neurobasal+B27+N2+bFGF+EGF?

Neurospheres can be plated on laminin coated culture plates for neuron differentiation. The issue is that it is difficult to control the plating density of neurospheres. Alternatively, neurospheres can be dissociated into single cells and plate single cell suspension at a certain density such as 1-5 x 10^4 cells/cm2 onto laminin coated plates for neuron differentiation. For general neuron differentiation, Neurobasal+B27+N2 can be used. Growth factors such as BDNF and/or GDNF can be added into medium for improving survival of differentiating NSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Neurobasal™ Medium FAQs

인용 및 참조 문헌 (5)

인용 및 참조 문헌
Abstract
A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.
Authors:Romito E,Battistella I,Plakhova V,Paplekaj A,Forastieri C,Toffolo E,Musio C,Conti L,Battaglioli E,Rusconi F
Journal:Journal of neuroscience methods
PubMed ID:39053772
Activation of Trk neurotrophin receptor signaling by pituitary adenylate cyclase-activating polypeptides.
Authors: Lee Francis S; Rajagopal Rithwick; Kim Albert H; Chang Paul C; Chao Moses V;
Journal:J Biol Chem
PubMed ID:11784714
'Pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide that acts through G protein-coupled receptors, exerts neuroprotective effects upon many neuronal populations. However, the intracellular signaling mechanisms that account for PACAP''s trophic effects are not well characterized. Here we have tested the possibility that PACAP uses neurotrophin signaling pathways. We have found ... More
Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.
Authors: Lesuisse Christian; Martin Lee J;
Journal:J Neurobiol
PubMed ID:11920724
A long-term cell culture system was used to study maturation, aging, and death of cortical neurons. Mouse cortical neurons were maintained in culture in serum-free medium (Neurobasal supplemented with B27) for 60 days in vitro (DIV). The levels of several proteins were evaluated by immunoblotting to demonstrate that these neurons ... More
Axonal Transport Enables Neuron-to-Neuron Propagation of Human Coronavirus OC43.
Authors:Dubé M, Le Coupanec A, Wong AHM, Rini JM, Desforges M, Talbot PJ
Journal:J Virol
PubMed ID:29925652
Human coronaviruses (HCoVs) are recognized respiratory pathogens for which accumulating evidence indicates that in vulnerable patients the infection can cause more severe pathologies. HCoVs are not always confined to the upper respiratory tract and can invade the central nervous system (CNS) under still unclear circumstances. HCoV-induced neuropathologies in humans are ... More
A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids.
Authors:Yang L, Han Y, Nilsson-Payant BE, Gupta V, Wang P, Duan X, Tang X, Zhu J, Zhao Z, Jaffré F, Zhang T, Kim TW, Harschnitz O, Redmond D, Houghton S, Liu C, Naji A, Ciceri G, Guttikonda S, Bram Y, Nguyen DT, Cioffi M, Chandar V, Hoagland DA, Huang Y, Xiang J, Wang H, Lyden D, Borczuk A, Chen HJ, Studer L, Pan FC, Ho DD, tenOever BR, Evans T, Schwartz RE, Chen S
Journal:Cell Stem Cell
PubMed ID:32579880
SARS-CoV-2 has caused the COVID-19 pandemic. There is an urgent need for physiological models to study SARS-CoV-2 infection using human disease-relevant cells. COVID-19 pathophysiology includes respiratory failure but involves other organ systems including gut, liver, heart, and pancreas. We present an experimental platform comprised of cell and organoid derivatives from ... More