StemPro™ Accutase™ Cell Dissociation Reagent

StemPro Accutase reagent is a gentle cell dissociation solution used for the detaching of cells while maintaining high cell viability as compared to animal-origin enzymes such as trypsin. It does not contain mammalian- or bacterial-derived products. Cell lines tested for StemPro Accutase reagent application include human embryonic stem cells, human mesenchymal stem cells, human neural stem cells, and primary cells.

StemPro Accutase Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex, CELLStart, and polymers.

Maintains high cell viability

StemPro Accutase reagent performs well in detaching primary and stem cells while maintaining high cell viability compared to animal origin enzymes such as trypsin. Some downstream applications following treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.

Detaches various cell types

Cell lines tested for StemPro Accutase reagent application include human embryonic stem cells (hESCs), human mesenchymal stem cells (hMSCs), human neural stem cells (hNSCs), and primary cells including macrophages, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, and established cell lines such as adherent CHO and BHK cells and 293 cells.