인용 및 참조 문헌 (26)

Invitrogen™

Phalloidin Labeling Probes

Name: Phalloidin Labeling Probes
SKU: A22284
Price: 972000 KRW

Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.

보기 방식 변경

카탈로그 번호	색상	여기 파장 범위	염료 유형
A22284	Far-red	632/647 nm	Alexa Fluor™ 633
A22281	Blue	346/442 nm	Alexa Fluor™ 350
A30104	Violet	405/450 nm	Alexa Fluor™ Plus 405
A12379	Green	495/518 nm	Alexa Fluor™ 488
O7466	Green	496/520 nm	Oregon Green™ 488
F432	Green	496/516 nm	FITC (Fluorescein)
A22282	Yellow	531/554 nm	Alexa Fluor™ 532
R415	Red-orange	540/565 nm	TRITC
A22283	Orange	556/570 nm	Alexa Fluor™ 546
A34055	Orange	555/565 nm	Alexa Fluor™ 555
A30106	Orange	555/565 nm	Alexa Fluor Plus 555
B3475	Red	558/569 nm	BODIPY™
A12380	Orange-red	578/600 nm	Alexa Fluor™ 568
A12381	Red	581/609 nm	Alexa Fluor™ 594
T7471	Red	591/608 nm	Texas Red™
A34054	Far-red	633/647 nm	Alexa Fluor™ 635
A22287	Far-red	650/668 nm	Alexa Fluor™ 647
A30107	Far-red	650/668 nm	Alexa Fluor Plus 647
A22285	Near-infrared	663/690 nm	Alexa Fluor™ 660
A22286	Near-infrared	679/702 nm	Alexa Fluor™ 680
A30105	Near-infrared	758/784 nm	Alexa Fluor™ Plus 750
B7474	None	None	Biotin-XX
P3457	None	None	Phalloidin (unlabeled)

23 개 옵션

카탈로그 번호 A22284

제품 가격(KRW)

972,000

Each

색상:

Far-red

여기 파장 범위:

632/647 nm

염료 유형:

Alexa Fluor™ 633

제품 가격(KRW)

972,000

Each

Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.

A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.

Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.

Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining

Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.

Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.

Features of phalloidin probes

High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
Ease of use—staining is straightforward and quick
Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton

For Research Use Only. Not for use in diagnostic procedures.

사양

색상Far-red

염료 유형Alexa Fluor™ 633

여기 파장 범위632/647 nm

용도(장비)Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible Cy5 filter set

제품라인Alexa Fluor

수량300 Units

배송 조건Room Temperature

라벨 유형Alexa Fluor Dyes

제품 유형Phalloidin

Sub Cellular LocalizationActin, Cytoskeleton

Unit SizeEach

구성 및 보관

Store in freezer -5°C to -30°C and protect from light.

자주 묻는 질문(FAQ)

Do you offer alternative package sizes for phalloidin or ReadyProbes reagents?

Alternative package sizes may be available through a custom request process. Contact Thermo Fisher Scientific customer support or your sales representative to discuss available options for the specific reagent and quantity needed.

Can I use conjugated phalloidin label probes on organoids?

Yes. Conjugated phalloidin labeling probes can be used with organoids when F-actin is accessible after fixation and permeabilization. Because organoids are thicker than monolayer cell cultures, protocol optimization may be needed, including permeabilization conditions, probe incubation time, and probe concentration.

Are conjugated phalloidin reagents antibodies?

No, Phalloidin is not an antibody. It is a bicyclic peptide toxin originally isolated from the Amanita phalloides mushroom and is used to bind and label F-actin in fixed and permeabilized samples.

Can I use methanol instead of DMSO to reconstitute the conjugated phalloidins from the lyophilized solids?

Yes, methanol can be used to reconstitute fluorescent phalloidin, but it creates a less concentrated stock solution than DMSO. Dissolve the vial contents in 1.5 mL of methanol to yield a 40X stock solution at a concentration of 200 assays/mL, which is equivalent to approximately 6.6 µM. One unit/assay of fluorescent phalloidins is equivalent to 5 µL of the methanolic stock solution. Methanol may be used as a stock-solution solvent, but methanol-containing fixatives are not recommended because they can disrupt F-actin.

How do I reconstitute fluorescent conjugated phalloidin probes to make a stock solution?

For fluorescent phalloidin label probes, prepare a DMSO stock solution by dissolving the vial contents in 150 µL anhydrous DMSO to make a 400X stock, with a concentration of approximately 66 µM or 2,000 assays per mL. Store between -25°C and -5°C, protected from light. One unit/assay of fluorescent phalloidins is equivalent to 0.5 µL of the DMSO stock solution.

View all Phalloidin Labeling Probes FAQs

인용 및 참조 문헌 (26)

인용 및 참조 문헌

Abstract

LMP4 regulates Tbx5 protein subcellular localization and activity.

Authors:Camarata T, Bimber B, Kulisz A, Chew TL, Yeung J, Simon HG

Journal:J Cell Biol

PubMed ID:16880269

'The limb- and heart-specific Tbx5 transcription factor coexpresses with and directly binds to the novel PDZ-LIM domain protein, LMP4. LMP4 is distributed in the cytoplasm associated with the actin cytoskeleton. In the presence of LMP4, Tbx5 shuttles dynamically between the nucleus and cytoplasm and, in a complex with LMP4, localizes ... More

Multiphoton excitation spectra in biological samples.

Authors:Dickinson ME, Simbuerger E, Zimmermann B, Waters CW, Fraser SE

Journal:J Biomed Opt

PubMed ID:12880336

'Multiphoton microscopy is becoming a popular mode of live and fixed cell imaging. This mode of imaging offers several advantages due to the fact that fluorochrome excitation is a nonlinear event resulting in excitation only at the plane of focus. Multiphoton excitation is enhanced by the use of ultrafast lasers ... More

Paper-supported 3D cell culture for tissue-based bioassays.

Authors:Derda R, Laromaine A, Mammoto A, Tang SK, Mammoto T, Ingber DE, Whitesides GM,

Journal:Proc Natl Acad Sci U S A

PubMed ID:19846768

Fundamental investigations of human biology, and the development of therapeutics, commonly rely on 2D cell-culture systems that do not accurately recapitulate the structure, function, or physiology of living tissues. Systems for 3D cultures exist but do not replicate the spatial distributions of oxygen, metabolites, and signaling molecules found in tissues. ... More

MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and proper PKA signal transduction.

Authors:Harada A, Teng J, Takei Y, Oguchi K, Hirokawa N

Journal:J Cell Biol

PubMed ID:12163474

Microtubule-associated protein 2 (MAP2) is a major component of cross-bridges between microtubules in dendrites, and is known to stabilize microtubules. MAP2 also has a binding domain for the regulatory subunit II of cAMP-dependent protein kinase (PKA). We found that there is reduction in microtubule density in dendrites and a reduction ... More

Targeting of calcium:calmodulin signals to the cytoskeleton by IQGAP1.

Authors:Psatha MI, Razi M, Koffer A, Moss SE, Sacks DB, Bolsover SR

Journal:Cell Calcium

PubMed ID:17137623

Mast cells reorganize their actin cytoskeleton in response to cytosolic calcium signals while in parallel secreting histamine and other inflammatory mediators. The effect of calcium on actin is mediated in large part through calmodulin. EGFP-tagged calmodulin is concentrated in the actin-rich cortex of RBL-2H3 mast cells. Transfection with small interfering ... More

View all Phalloidin Labeling Probes citations