RevitaCell™ Supplement (100X)
RevitaCell™ Supplement (100X)
Gibco™

RevitaCell™ Supplement (100X)

RevitaCell™ Supplement (100X) has been optimized for use with pluripotent stem cells (PSCs), either as a post-thaw recovery solution to자세히 알아보기
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카탈로그 번호수량
A26445015 mL
카탈로그 번호 A2644501
제품 가격(KRW)
280,000
キャンペーン価格
Ends: 31-Dec-2025
311,000
할인액 31,000 (10%)
Each
카트에 추가하기
수량:
5 mL
제품 가격(KRW)
280,000
キャンペーン価格
Ends: 31-Dec-2025
311,000
할인액 31,000 (10%)
Each
카트에 추가하기
RevitaCell™ Supplement (100X) has been optimized for use with pluripotent stem cells (PSCs), either as a post-thaw recovery solution to improve cell viability or in combination with Essential 8™ Medium to enhance single cell passaging applications. It can also be used for recovery of primary neurons, traditionally a challenging cell to efficiently recover. The significant improvement seen in these applications is due to the presence of a more specific ROCK inhibitor coupled with molecules containing antioxidant and free radical scavenger properties that minimize the impact of stress on the cells.

• Enabling—provides maximum cell viability when performing single cell passaging
• Rapid cell recovery—high post-thaw cell viability and growth of pluripotent stem cells (passaged as clumps or single cells) and neurons
• Consistent—formulated using animal origin-free and chemically defined materials for quality and confidence

Single cell passaging
Use of the RevitaCellTM Supplement for 18-24 hours post-single cell passaging provides vast improvement in cell survival. Cells are shown to reach passaging confluency within 3-4 days post-seeding.

Higher specificity
Kinase profiling of pro-survival small molecules in RevitaCell™ Supplement shows higher specificity in comparison to traditional ROCK inhibitors (i.e., Y-27632 and Thiazovivin).

Quicker post-thaw recovery
PSCs recovered at 40,000 viable cells/cm2 using media supplemented with RevitaCell™ Supplement for the first 24 hours post-thaw recovery followed by feeding with unsupplemented Essential 8™ Medium will typically reach 80% confluency in <5 days, significantly reducing the average post-thaw recovery time of most cryopreservation solutions. Neurite outgrowth of neurons is also significantly improved using RevitaCell™ Supplement.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Stem Cells (iPS - Induced Pluripotent Stem)
농도100 X
배양 유형Stem Cell (Embryonic, iPS - Induced Pluripotent Stem)
제조 품질cGMP for medical devices, 21 CFR Part 820 and ISO 13485
제품라인Gibco, RevitaCell
수량5 mL
배송 조건Dry Ice
소스Animal origin-free
분류Animal Origin-free
제품 유형Media Supplement
Unit SizeEach
구성 및 보관
Store at -5 to -20°C and protect from light.

자주 묻는 질문(FAQ)

Can RevitaCell Supplement be used in addition to a traditional ROCK Inhibitor?

No. Combination of RevitaCell Supplement with a traditional ROCK inhibitor will result in deleterious effects on PSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The RevitaCell Supplement in the PSC Cryopreservation Kit has a strong smell. Is this normal?

Yes, it is normal that the RevitaCell Supplement has a strong smell.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between the PSC Cryopreservation Kit and the CTS PSC Cyropreservation Kit?

The CTS PSC Cryopreservation Kit (Cat. No. A4239301) provides the same efficient recovery, consistency, and reduced variability as the PSC Cryopreservation Kit (Cat. No. A2644601). However, these two kits have different intended use statements, i.e., “For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals” and “For Research Use Only”, respectively. Furthermore, the CTS PSC Cryopreservation Kit has full USP sterility testing info and a Drug Master file (DMF), whereas the sterility testing of the PSC Cryopreservation is via a modified USP method, and it does not have a DMF.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is StemFlex Medium compatible with downstream differentiation kits available from Thermo Fisher Scientific?

Yes. We have seen compatibility with the following differentiation kits provided by Thermo Fisher Scientific: PSC Cardiomyocyte Differentiation Kit (Cat. No. A2921201), PSC Definitive Endoderm Induction Kit (Cat. No. A3062601), PSC Neural Induction Medium (Cat. No. A1647801), and PSC Dopaminergic Neuron Differentiation Kit (Cat. No. A3147701).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What protocol is recommended for delivery of Cas9 protein:guide RNA complex to my PSCs cultured in StemFlex Medium?

We recommend use of the Neon Electroporation device for electroporation of PSCs with Cas9 protein:guide RNA complex following the protocol guidance in the following demonstrated protocol (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/PG1584-PJT1313-COL31227-Demonstrated-Protocol-Performing-CRISPR-Cas9-FHR.pdf); see the section entitled “Knockout by electroporation of RNP using the Neon Transfection System.” We have seen that the Neon electroporation protocols 7 and 14 provide optimal indel formation while maintaining cell survival. However, the electroporation conditions may need to be optimized for your pluripotent cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.