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인용 및 참조 문헌 (10)
Gibco™
StemFlex™ Medium
StemFlex Medium enables long term feeder-free culture of pluripotent stem cells (PSCs) and is optimized to support challenging applications, including single-cell passaging, gene editing, and reprogramming.
| 카탈로그 번호 | 수량 |
|---|---|
| A3349401 | 500 mL |
카탈로그 번호 A3349401
제품 가격(KRW)
533,000
온라인 행사
Ends: 31-Dec-2025
592,000할인액 59,000 (10%)
Each
수량:
500 mL
제품 가격(KRW)
533,000
온라인 행사
Ends: 31-Dec-2025
592,000할인액 59,000 (10%)
Each
StemFlex Medium's unique formulation supports robust expansion of PSCs while offering the convenience of a flexible feeding schedule (including weekend-free options) and the ability to choose the matrix and passaging reagent that best suits specific applications. StemFlex Medium enables the long term feeder-free culture of PSCs without karyotypic abnormalities and maintains the cells' ability to differentiate into all three germ layers.
StemFlex Medium:
- Delivers superior results in challenging applications
- Offers flexibility for PSC culture conditions and feeding schedules
- Provides exceptional value and performance
Superior results
StemFlex Medium is formulated to deliver superior outcomes in challenging applications that stress PSC cultures, such as reprogramming, single cell passaging, and gene editing. It provides superior cell survival compared to a leading competitor (mTeSR1), allowing for greater success in the most relevant cell types.
Flexibility
StemFlex Medium allows for unprecedented three-way usage flexibility for PSC culture conditions: the ability to select an optimal feeding schedule, including a weekend-free option; a choice of matrices, depending upon the needs of the workflow; and the option to select the passaging reagent for the level of cell dissociation required.
Exceptional value and performance
StemFlex Medium is provided in a convenient two-component kit (450 mL basal medium & 50 mL supplement) and, when used with Geltrex Flex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix, provides a cost-effective, robust system for superior feeder-free culture of human PSCs. StemFlex Medium's ability to enable weekend-free culture of PSCs provides unmatched value among the robust feeder-fee PSC media on the market today.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포주Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
세포 유형Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
농도StemFlex Basal Medium (1X); StemFlex Supplement (10X)
제조 품질cGMP for medical devices, 21 CFR Part 820 and ISO 13485
제품라인StemFlex
제품 유형Stem Cell Media
수량500 mL
유통 기한12 Months
배송 조건Supplement ships on dry ice, basal can ship at RT
분류Animal Origin-free
Culture TypeAdherent Cell Culture, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
형태Liquid
Serum LevelSerum-free
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함Phenol Red
Unit SizeEach
구성 및 보관
• 450 mL Basal Medium, store at 2–8°C and protect from light
• 50 mL Supplement, store at -5 to -20°C and protect from light
• 50 mL Supplement, store at -5 to -20°C and protect from light
자주 묻는 질문(FAQ)
What is the glucose content of the StemFlex Medium (Cat. No. A3349401)?
What media do you recommend for standard single-cell cloning of iPSCs?
Does StemFlex Medium contain pen/strep antibiotic?
What is the difference between StemFlex Medium and Essential 8 Flex Medium?
Has StemFlex Medium been evaluated for growth of mouse ESCs?
인용 및 참조 문헌 (10)
인용 및 참조 문헌
Abstract
Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media.
Journal:iScience
PubMed ID:30267684
'We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells
Improving single-cell cloning workflow for gene editing in human pluripotent stem cells.
Journal:Stem Cell Res
PubMed ID:30099335
'The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens. Unfortunately, the procedures for genome editing of hPSCs often subject cells to harsh conditions that compromise viability: a major problem that is compounded by
Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing.
Journal:Cell Rep
PubMed ID:32460021
'CRISPR genome editing is a promising tool for translational research but can cause undesired editing outcomes, both on target at the edited locus and off target at other genomic loci. Here, we investigate the occurrence of deleterious on-target effects (OnTEs) in human stem cells after insertion of disease-related mutations by
Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells.
Journal:Sci Rep
PubMed ID:32873852
'Hepatocytes derived from human iPSCs are useful to study hepatitis B virus (HBV) infection, however infection efficiency is rather poor. In order to improve the efficiency of HBV infection to iPSC-derived hepatocytes, we set a co-culture of hepatocytes with liver non-parenchymal cells and found that liver sinusoidal endothelial cells (LSECs)
A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons.
Journal:Sci Rep
PubMed ID:31227747
'Neurons created from human induced pluripotent stem cells (hiPSCs) provide the capability of identifying biological mechanisms that underlie brain disorders. IPSC-derived human neurons, or iNs, hold promise for advancing precision medicine through drug screening, though it remains unclear to what extent iNs can support early-stage drug discovery efforts in industrial-scale