Pierce™ DSG, No-Weigh™ Format
Pierce™ DSG, No-Weigh™ Format
Thermo Scientific™

Pierce™ DSG, No-Weigh™ Format

Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface자세히 알아보기
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카탈로그 번호수량
A3539210 x 1 mg
2059350 mg
카탈로그 번호 A35392
제품 가격(KRW)
216,000
Online offer
Ends: 31-Dec-2025
240,000
할인액 24,000 (10%)
Each
카트에 추가하기
수량:
10 x 1 mg
제품 가격(KRW)
216,000
Online offer
Ends: 31-Dec-2025
240,000
할인액 24,000 (10%)
Each
카트에 추가하기
Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

Features of disuccinimidyl glutarate:

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)
• Membrane-permeable, allowing for intracellular crosslinking
• Can increase crosslinking efficiency compared to DSS in some applications

Product References
Crosslinker Application Guide -- search for recent literature references for this product

For Research Use Only. Not for use in diagnostic procedures.
사양
세포 투과성Yes
형태Powder
라벨링 방법Chemical Labeling
분자량326.26
페길레이티드No
제품라인No-Weigh, Pierce
수량10 x 1 mg
반응성 부분NHS Ester
배송 조건Wet Ice
용해도DMF, DMSO
스페이서 암 길이7.7 Å
수용성No
화학물질 반응성Amine-Amine
CleavableNo
교차결합제 유형Homobifunctional
형식Standard, Single-use
제품 유형Crosslinker
스페이서Short (<10 Å)
Unit SizeEach
구성 및 보관
Upon receipt store desiccated at 2 to 8°C.

자주 묻는 질문(FAQ)

Can you provide the shelf-life for DSG (disuccinimidyl glutarate)?

DSG (disuccinimidyl glutarate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How should I dissolve and store DSG, No-Weigh Format?

DSG is an NHS-ester that hydrolyzes easily and is moisture-sensitive. For optimal results, we recommend storing DSG powder desiccated at 4 degrees C. To avoid moisture condensation onto the product, the vial must be equilibrated to room temperature before opening. We recommend resuspending DSG in DMSO, Anhydrous (Cat. No. D12345) right before use and not storing the solution.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How does DSP work as a crosslinker?

DSP is a homobifunctional, water soluble, cleavable, amine-reactive cross-linker. The amine-reactive ends are NHS-esters. This group reacts with primary amines, in proteins that translates to lysine groups and N-terminus amines. It reacts best in the pH range of 7.5 to 8.5, this ensures the amines are deprotonated and it limits the hydrolysis of the ester at higher pHs. NHS-esters are best used in a heavy molar excess over the number of amines present. This helps to overcome the competing hydrolysis of the ester to an inactive form.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How is the DSP cross-linking reaction quenched?

1M Tris (or glycine), pH 7.5 can be added to the reaction mixture to a final concentration of approximately 20-50 mM.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How is DSP cleaved?

The disulfIde bond in DSP's 12Å spacer arm can be cleaved by at least three different methods. The first is by incubating the crosslinker in 10-50 mM DTT for 30 minutes at 37 degrees C. Secondly, the disulfIde can be reduced by incubation in 5% 2-Mercaptoethanol (2-ME) in SDS-PAGE sample buffer at 100 degrees C for 5 minutes. Finally, the “-S-S-” bond can be cleaved by incubation in 100 mM sodium borohydride at room temperature for 30-60 minutes. Other reducing agents such as TCEP-HCl or 2-Mercaptoethylamine (2-MEA) should also work, however these applications have not been referenced.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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