Bolt LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris Plus gels. It자세히 알아보기
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10 mL
제품 가격(KRW)
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Ends: 31-Dec-2025
30,000
할인액 2,000 (7%)
Each
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Bolt LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris Plus gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Bolt LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.
To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results.
Note: Bolt LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. Bolt LDS Sample Buffer also contains a higher concentration glycerol.
For Research Use Only. Not for use in diagnostic procedures.
사양
버퍼Sample Loading Buffers
농도4X
젤 호환성Bolt™ Bis-Tris Plus Gels
제품라인Bolt
제품 유형LDS Sample Buffer
수량10 mL
용도 (장비)Bolt Mini Gel Tank
젤 유형Bis-Tris Plus
Unit SizeEach
구성 및 보관
Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red.
Store at 4°C to 25°C.
자주 묻는 질문(FAQ)
Can I prepare my protein sample with the reducing agent and store it for future use?
DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.
My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?
Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.
While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.