CyQUANT™ Cell Proliferation Assays
CyQUANT™ Cell Proliferation Assays
Invitrogen™

CyQUANT™ Cell Proliferation Assays

CyQUANT™ NF Cell Proliferation Assay Kit는 빠르고 민감하게 군집 내 세포를 계측하고 마이크로플레이트 형식에서 증식을 측정할 수 있는 방법을 제공합니다.•자세히 알아보기
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카탈로그 번호세포 유형수량
C35006NF Cell1000 Assays
C35011Direct Cell10 Microplates
C35013Direct Red Cell10 Microplates
C35012Direct Cell100 Microplates
C35007NF Cell200 Assays
C7026For cells in culture1000 Assays
C7027Cell Lysis Buffer50 mL
카탈로그 번호 C35006
제품 가격(KRW)
628,000
Online offer
Ends: 31-Mar-2026
717,000
할인액 89,000 (12%)
Each
카트에 추가하기
세포 유형:
NF Cell
수량:
1000 Assays
제품 가격(KRW)
628,000
Online offer
Ends: 31-Mar-2026
717,000
할인액 89,000 (12%)
Each
카트에 추가하기
CyQUANT™ NF Cell Proliferation Assay Kit는 빠르고 민감하게 군집 내 세포를 계측하고 마이크로플레이트 형식에서 증식을 측정할 수 있는 방법을 제공합니다.
• MTT 또는 AlamarBlue™ assay 보다 민감
• 선형 검출 범위 100 ∼ 20,000 cell/well (96-well microplate)
• 1시간 내에 분석을 완료할 수 있습니다.

빠르고 간편한 세포 증식 측정 방법
CyQUANT™ NF Cell Proliferation assay는 세포 용해, 긴 배양, 방사능, 세포 염색 제거 등이 필요하지 않습니다. CyQUANT™ NF assay는 세포막 투과 시약과 함께 세포 투과성 DNA 결합 dye를 사용하여 기존 CyQUANT™ 세포 증식 분석과 달리 동결-해동 세포 용해 단계가 필요하지 않습니다. CyQUANT™ NF Cell Proliferation Assay Kit는 96-well 또는 384-well 마이크로플레이트 형식으로 사용할 수 있으며 다음 두 가지 구성으로 제공됩니다. 샘플 크기가 작을 때에는 200 assay kit (C35007), high-throughput 어플리케이션에는 1000 assay kit (C35006)를 사용하십시오.

AlamarBlue™는 TREK Diagnostic Systems의 등록 상표입니다.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형NF Cell
검출 방법Fluorescence
염료 유형Other Label(s) or Dye(s)
여기/방출480/520 nm
형식384-well plate, 96-well plate
수량1000 Assays
배송 조건Room Temperature
용도(애플리케이션)Proliferation Assay
용도 (장비)Microplate Reader, HTS Reader
제품라인CyQUANT
제품 유형Cell Proliferation Assay
Unit SizeEach
구성 및 보관
•CyQUANT™ NF dye reagent
•Dye delivery reagent
•5X HBSS buffer (Hank’s balanced salt solution)

It is possible to observe precipitate in HBSS buffer. The precipitate does not have an impact on the use of this kit, nor the results that are gathered from its use.

자주 묻는 질문(FAQ)

What is the difference between the original CyQuant assay and the CyQuant NF assay?

The original CyQUANT assay provides sensitive detection of cells over a 1000-fold linear dynamic range. In this assay, a freeze-thaw cell lysis step is required to facilitate the interaction of the CyQUANT GR dye with DNA. The CyQUANT NF assay avoids this freeze-thaw step by using a DNA binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT NF protocol requires only aspiration of growth medium (for adherent cells), replacement with dye binding solution, incubation for 30-60 minutes, and then measurement of fluorescence in a microplate reader. The assay is designed to produce a linear analytical response from at least 100-20,000 cells per well in most cell lines in a 96-well microplate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you have spectral data for the CyQUANT NF Cell Proliferation Assay kit?

When CyQUANT NF dye is bound to DNA, the approximate excitation/emission is 480/520 nm. It can be detected using standard GFP or FITC filter settings.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (15)

인용 및 참조 문헌
Abstract
KIF14 messenger RNA expression is independently prognostic for outcome in lung cancer.
Authors:Corson TW,Zhu CQ,Lau SK,Shepherd FA,Tsao MS,Gallie BL
Journal:Clinical cancer research : an official journal of the American Association for Cancer Research
PubMed ID:17545527
Functional symbiosis between endothelium and epithelial cells in glomeruli.
Authors:Hirschberg R, Wang S, Mitu GM,
Journal:Cell Tissue Res
PubMed ID:17999087
'In some capillary beds, pericytes regulate endothelial growth. Capillaries with high filtration capacity, such as those in renal glomeruli, lack pericytes. Glomerular endothelium lies adjacent to visceral epithelial cells (podocytes) that are anchored to and cover the anti-luminal surface of the basement membrane. We have tested the hypothesis that podocytes ... More
Flex-Hets differentially induce apoptosis in cancer over normal cells by directly targeting mitochondria.
Authors:Liu T, Hannafon B, Gill L, Kelly W, Benbrook D
Journal:Mol Cancer Ther
PubMed ID:17575110
'Flex-Het drugs induce apoptosis in multiple types of cancer cells, with little effect on normal cells. This apoptosis occurs through the intrinsic mitochondrial pathway accompanied by generation of reactive oxygen species (ROS). The objective of this study was to determine if direct or indirect targeting of mitochondria is responsible for ... More
Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-responsive core-shell nanoparticles.
Authors:Hu Y, Litwin T, Nagaraja AR, Kwong B, Katz J, Watson N, Irvine DJ,
Journal:Nano Lett
PubMed ID:17887715
'Polycations that absorb protons in response to the acidification of endosomes can theoretically disrupt these vesicles via the "proton sponge" effect. To exploit this mechanism, we created nanoparticles with a segregated core-shell structure for efficient, noncytotoxic intracellular drug delivery. Cross-linked polymer nanoparticles were synthesized with a pH-responsive core and hydrophilic ... More
Decrease of endogenous vascular endothelial growth factor may not affect glioma cell proliferation and invasion.
Authors:Hong X, Jiang F, Kalkanis SN, Zhang ZG, Zhang X, Zheng X, Mikkelsen T, Jiang H, Chopp M
Journal:J Exp Ther Oncol
PubMed ID:17552362
'Vascular endothelial growth factor (VEGF) is abundantly produced by glioma cells especially glioblastoma, the most malignant form of astrocytoma. VEGF, a well known angiogenic factor, acts in a paracrine fashion on endothelial cells to develop tumor vasculature. However, recent studies have found that several tumor cells express VEGF receptors, and ... More