TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기

TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent <i>E. coli</i> cells

인용 및 참조 문헌 (49)

Invitrogen™

TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

Name: TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells
SKU: K460001
Price: 877000 KRW

빠르고 효율적인 클로닝과 차후 in vitro transcription⁄translation을 위해서는 Invitrogen's TOPO™ TA cloning Dual Promoter Kit와 본사의 인기 제품인 pCR™II vector를자세히 알아보기

보기 방식 변경

카탈로그 번호	수량
K460001	25 Reactions
K4600J10	10 Reactions
K460040	50 Reactions

3 개 옵션

카탈로그 번호 K460001

제품 가격(KRW)

877,000

Online offer

Ends: 31-Dec-2025

1,031,000

할인액 154,000 (15%)

Each

카트에 추가하기

수량:

25 Reactions

제품 가격(KRW)

877,000

Online offer

Ends: 31-Dec-2025

1,031,000

할인액 154,000 (15%)

Each

카트에 추가하기

빠르고 효율적인 클로닝과 차후 in vitro transcription⁄translation을 위해서는 Invitrogen's TOPO™ TA cloning Dual Promoter Kit와 본사의 인기 제품인 pCR™II vector를 함께 사용하는 것이 탁월한 선택입니다. 시간이 많이 걸리고 지루한 restriction site cloning 과정을 없앤 TOPO™ cloning은 믿을 수 있는 최고의 방법으로 3단계 프로토콜을 사용해 단 5분 만에 최고 95% recombinant를 수득할 수 있습니다.

TOPO™ TA Dual Promoter Kit와 본사의 pCR™II-TOPO™ TA vector 병용은 다음과 같은 편리한 특징을 갖습니다.

• 3′-T overhangs - Taq-amplified PCR 산물의 direct ligation
• T7 및 SP6 promoters - 효율적인 in vitro transcription
• M13 forward/reverse primer site - sequencing
• EcoR I franking insert를 포함한 편리하고 검증된 restriction site 16개 - 차후 절단 또는 sub-cloning
• 선택한 E. coli에 대한 Kanamycin 및 ampicillin resistance
• 용이한 blue⁄white colony screening - recombinant 선택
• OneShot™ TOP10 Competent cells이 포함 - 편의성과 최고의 cloning 효율을 보장함

For Research Use Only. Not for use in diagnostic procedures.

사양

박테리아 또는 효모 균주TOP10

세포 유형Chemically Competent

클로닝 방법TOPO™-TA

제품라인One Shot

제품 유형Cloning Kit

프로모터T7, SP6

수량25 Reactions

벡터TOPO-TA Cloning Vectors

형식Kit

Unit SizeEach

구성 및 보관

Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.

자주 묻는 질문(FAQ)

Can I store my competent E. coli in liquid nitrogen?

We do not recommend storing competent E. coli strains in liquid nitrogen as the extreme temperature can be harmful to the cells. Also, the plastic storage vials are not intended to withstand the extreme temperature and may crack or break.

How should I store my competent E. coli?

We recommend storing our competent E. coli strains at -80°C. Storage at warmer temperatures, even for a brief period of time, will significantly decrease transformation efficiency.

What is the difference between the pCR2.1-TOPO and pCR4-TOPO vectors?

The vector backbones for both of these vectors are very similar. The main difference is that the pCR4-TOPO vector has sequencing primer sites located as close as 33 base pairs from the PCR product insertion site. This minimizes the amount of vector DNA sequence that needs to be read before reaching the sequence of the insert, making the pCR4-TOPO vector very useful for sequencing applications.

What is the difference between the pCR2.1-TOPO and pCRII-TOPO vectors?

The vector backbones for both of these vectors are very similar. The main difference is that the pCRII-TOPO vector is a dual promoter vector, containing the SP6 and T7 promoters for in vitro transcription/sequencing, whereas the pCR2.1-TOPO vector contains only the T7 promoter for in vitro transcription/sequencing. Both vectors contain the M13 Forward and Reverse primer sites for sequencing or PCR screening.

Your TOPO cloning kits contain a control template and control primers. Can I obtain the sequence of the control template?

The sequence of the control template is proprietary.

View all TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells, 25 Reactions FAQs

인용 및 참조 문헌 (49)

인용 및 참조 문헌

Abstract

Cloning and functional expression of a thyrotropin receptor cDNA from rat fat cells.

Authors:Endo T; Ohta K; Haraguchi K; Onaya T;

Journal:J Biol Chem

PubMed ID:7738021

Thyrotropin receptor (TSH-R) has been thought to be thyroid-specific, but, by Northern blot analysis, we found that rat adipose tissue expressed TSH-R mRNAs in amounts approaching those in the thyroid. To investigate the function of TSH-R from adipose tissue, we screened a rat fat cell lambda gt11 cDNA library for ... More

Modulation of mouse Paneth cell alpha-defensin secretion by mIKCa1, a Ca2+-activated, intermediate conductance potassium channel.

Authors: Ayabe Tokiyoshi; Wulff Heike; Darmoul Dalila; Cahalan Michael D; Chandy K George; Ouellette Andre J;

Journal:J Biol Chem

PubMed ID:11724775

'Paneth cells in small intestinal crypts secrete microbicidal alpha-defensins in response to bacteria and bacterial antigens (Ayabe, T., Satchell, D. P., Wilson, C. L., Parks, W. C., Selsted, M. E., and Ouellette, A. J. (2000) Nat. Immunol. 1, 113- 38). We now report that the Ca(2+)-activated K(+) channel mIKCa1 modulates ... More

Variant forms of alpha-fetoprotein transcripts expressed in human hematopoietic progenitors. Implications for their developmental potential towards endoderm.

Authors: Kubota Hiroshi; Storms Robert W; Reid Lola M;

Journal:J Biol Chem

PubMed ID:12006569

'Hematopoietic stem cells have been identified as multipotent cells that give rise to all adult hematopoietic lineages. Although the hematopoietic lineage is derived from the mesodermal germ layer in the embryo, recent data suggest that bone marrow cells with an antigenic profile consistent with that of hematopoietic stem cells can ... More

Mutation of the matrix metalloproteinase At2-MMP inhibits growth and causes late flowering and early senescence in Arabidopsis.

Authors: Golldack Dortje; Popova Olga V; Dietz Karl-Josef;

Journal:J Biol Chem

PubMed ID:11726650

'This study characterizes the expression and functional significance of the member of the matrix metalloproteinase (MMP) family At2-MMP from Arabidopsis. By transcript analysis, expression of At2-MMP was found in leaves and roots of juvenile Arabidopsis and leaves, roots, and inflorescences of mature flowering plants showing strong increase of transcript abundance ... More

Phosphorylation of the Carboxyl Terminus of Inner Centromere Protein (INCENP) by the Aurora B Kinase Stimulates Aurora B Kinase Activity.

Authors: Bishop John D; Schumacher Jill M;

Journal:J Biol Chem

PubMed ID:12048181

'How the events of mitosis are coordinated is not well understood. Intriguing mitotic regulators include the chromosomal passenger proteins. Loss of either of the passengers inner centromere protein (INCENP) or the Aurora B kinase results in chromosome segregation defects and failures in cytokinesis. Furthermore, INCENP and Aurora B have identical ... More

View all TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells, 25 Reactions citations