MitoProbe™ Transition Pore Assay Kit, for flow cytometry

인용 및 참조 문헌 (16)

Invitrogen™

MitoProbe™ Transition Pore Assay Kit, for flow cytometry

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability자세히 알아보기

카탈로그 번호	수량
M34153	1 kit

카탈로그 번호 M34153

제품 가격(KRW)

836,000

Online offer

Ends: 31-Mar-2026

983,000

할인액 147,000 (15%)

카트에 추가하기

1 kit

제품 가격(KRW)

836,000

Online offer

Ends: 31-Mar-2026

983,000

할인액 147,000 (15%)

카트에 추가하기

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.

여기/방출488

반응 수100 Reactions

제품라인MitoProbe

제품 유형Transition Pore Assay Kit

수량1 kit

배송 조건Room Temperature

콘주게이트Calcein AM

형식Tube

Unit SizeEach

구성 및 보관

Contains 5 vials of calcein, AM (50 μg of dry powder/vial), 1 vial of cobalt (II) Chloride hexahydrate (1.2 mL, 80 mM in saline), 1 vial of ionomycin (55 μg), calcium salt, and 1 vial of DMSO (1.5 mL).

Store in freezer (-5 to -30°C).

자주 묻는 질문(FAQ)

What are the advantages of flow cytometry?

-Measures data from single cells.
-Data are obtained for a large number of cells, generating a rich statistical analysis of cell populations.
-Because single cells are measured, it will reveal heterogeneity within a population.
-With the ability to multiplex, small sub-populations can be identified.
-Thousands of cells can be analyzed rapidly.
-It is ideally suited for blood samples and other cells in suspension.
-Data can be re-analyzed multiple times after acquisition.
-Flow cytometry files (FCS) can be archived.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kinds of applications can I run on a flow cytometer?

There are several applications, some of which include immunophenotyping, cell cycle analysis, apoptosis assays such as annexin V staining, CellEvent Caspase-3/7 assay, and TUNEL assay, cell viability, proliferation assays such as CellTrace assay and Click-iT EdU assay, measurements of mitochondrial potential with MitoProbe assays, and cell counting using counting beads.

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

인용 및 참조 문헌 (16)

인용 및 참조 문헌

Abstract

Cysteine 203 of cyclophilin D is critical for cyclophilin D activation of the mitochondrial permeability transition pore.

Authors:Nguyen TT, Stevens MV, Kohr M, Steenbergen C, Sack MN, Murphy E,

Journal:J Biol Chem

PubMed ID:21930693

The mitochondrial permeability transition pore (mPTP) opening plays a critical role in mediating cell death during ischemia/reperfusion (I/R) injury. Our previous studies have shown that cysteine 203 of cyclophilin D (CypD), a critical mPTP mediator, undergoes protein S-nitrosylation (SNO). To investigate the role of cysteine 203 in mPTP activation, we ... More

Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence.

Authors:Petronilli V, Miotto G, Canton M, Brini M, Colonna R, Bernardi P, Di Lisa F

Journal:Biophys J

PubMed ID:9929477

'The occurrence and the mode of opening of the mitochondrial permeability transition pore (MTP) were investigated directly in intact cells by monitoring the fluorescence of mitochondrial entrapped calcein. When MH1C1 cells and hepatocytes were loaded with calcein AM, calcein was also present within mitochondria, because (i) its mitochondrial signal was ... More

Calpain and caspase orchestrated death signal to accomplish apoptosis induced by resveratrol and its novel analog hydroxystilbene-1 [correction of hydroxstilbene-1] in cancer cells.

Authors:Guha P, Dey A, Dhyani MV, Sen R, Chatterjee M, Chattopadhyay S, Bandyopadhyay SK,

Journal:J Pharmacol Exp Ther

PubMed ID:20484155

'Stomach ulceration is a major side effect of most chemopreventive drugs. We have established that although resveratrol is a promising chemopreventive compound, it delays the ulcer healing process. However, its analog hydroxystilbene-1 (HST-1) was devoid of such an ulcerogenic side effect. Consequently, here we tried to explore the chemopreventive efficacy ... More

Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts.

Authors:Cotán D, Cordero MD, Garrido-Maraver J, Oropesa-Ávila M, Rodríguez-Hernández A, Gómez Izquierdo L, De la Mata M, De Miguel M, Lorite JB, Infante ER, Jackson S, Navas P, Sánchez-Alcázar JA,

Journal:FASEB J

PubMed ID:21551238

Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) is a mitochondrial disease most usually caused by point mutations in tRNA genes encoded by mtDNA. Here, we report on how this mutation affects mitochondrial function in primary fibroblast cultures established from 2 patients with MELAS who harbored the A3243G mutation. Both ... More

Imaging the mitochondrial permeability transition pore in intact cells.

Authors:Petronilli V, Miotto G, Canton M, Colonna R, Bernardi P, Di Lisa F

Journal:Biofactors

PubMed ID:9914828

The involvement of mitochondrial permeability transition pore (MTP) in cellular processes is generally investigated by indirect means, such as changes in mitochondrial membrane potential or pharmacological inhibition. However, such effects could not be related univocally to MTP. In addition, source of errors could be represented by the increased retention of ... More

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