Novex™ Tris-Glycine Mini Protein Gels, 4–12%, 1.0 mm, WedgeWell™ format
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Novex™ Tris-Glycine Mini Protein Gels, 4–12%, 1.0 mm, WedgeWell™ format
Novex™ Tris-Glycine Mini Protein Gels, 4–12%, 1.0 mm, WedgeWell™ format
Novex™ Tris-Glycine Mini Protein Gels, 4–12%, 1.0 mm, WedgeWell™ format
Invitrogen™

Novex™ Tris-Glycine Mini Protein Gels, 4–12%, 1.0 mm, WedgeWell™ format

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity.
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XP04120BOX10-well10 gels (1 box)
XP04120PK210-well2 gels (1 box)
XP04122BOX12-well10 gels (1 box)
XP04125BOX15-well10 gels (1 box)
카탈로그 번호 XP04120BOX
제품 가격(KRW)
233,000
10 gels (1 box)
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웰:
10-well
수량:
10 gels (1 box)
제품 가격(KRW)
233,000
10 gels (1 box)
카트에 추가하기
Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Features of Novex Tris-Glycine gels, WedgeWell format, include:
Improved shelf life—store gels for up to 12 months at 4°C
Diversity—use for native and denaturing protein assays
Wedge-shaped wells—easily load up to two times more sample volume
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC26755). For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer (LC2673) and Novex Tris-Glycine Native Running Buffer (LC2672).

Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer (LC367) when preforming traditional wet transfer using the Invitrogen Mini Blot Module (B1000). Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

For Research Use Only. Not for use in diagnostic procedures.
사양
Gel Thickness1.0 mm
길이(미터법)8 cm
분리 모드Molecular Weight
제품라인Novex, WedgeWell
수량10 gels (1 box)
권장 애플리케이션Denaturing, Native
샘플 로딩 부피Up to 60 μL
유통 기한12 Months
배송 조건Wet Ice
보관 요구 사항Store at 2°C to 8°C. Do not freeze.
폭(미터법)8 cm
용도 (장비)Mini Gel Tank, XCell SureLock Mini-Cell
젤 비율4 to 12%
젤 크기Mini
젤 유형Tris-Glycine Plus
분리 범위20 to 250 kDa
분리 유형Denaturing, Native
10-well
Unit Size10 gels (1 box)

자주 묻는 질문(FAQ)

Is it okay to use protein gels past their expiration date?

We do not recommend using gels past their expiration date because over time, the polyacrylamide hydrolyzes to acrylic acid and ammonia and this will affect the resolution of the proteins. Breakdown of polyacrylamide matrix is identified by:
- Ghost bands and doublets, seen first in the high molecular weight proteins
- Smiling of dye front across the gel, with bands in outer lanes becoming very slanted - proteins run slower there due to change in pH and pore size over time.


Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I run your protein gels overnight?

This is not really recommended, but it is always possible to increase run time by lowering the voltage of the run. In general, the relationships are linear - i.e., decreasing voltage by half will double the run time.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Do I need to increase the voltage when I run a 1.5 mm protein gel versus a 1.0 mm gel?

If you are running the gel at constant voltage, you do not need to increase the voltage regardless of the thickness of the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why do you recommend running your protein gels at constant voltage?

Using constant voltage allows the current and power to decrease during the run, providing a safety margin in case of a break in the system. Having lower power is a safety benefit and will also decrease the chances of experiencing an overheating of the gel. Further, the constant voltage setting does not need adjustment to account for differences in number or thickness of gels being run.

Find additional tips, troubleshooting help, and resources within ourProtein Gel 1D Electrophoresis Support Center.

Can Novex WedgeWell gels be stored at room temperature?

No. Novex WedgeWell gels must be stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.