Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems

Citations & References (3428)

Applied Biosystems™

Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems

Notebook Computer Kit for the Applied Biosystems™ 7500 and 7300 Real-Time PCR Systems.Used With7500 Real-Time PCR System with Dell™ Notebook.ForRead more

Catalog Number	Quantity
4359286	1 kit

Catalog number 4359286

Price (MXN)

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Quantity:

1 kit

Notebook Computer Kit for the Applied Biosystems™ 7500 and 7300 Real-Time PCR Systems.

Used With
7500 Real-Time PCR System with Dell™ Notebook.

For Research Use Only. Not for use in diagnostics procedures.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)Real Time PCR (qPCR)

For Use With (Equipment)7300 System, 7500 Fast System, 7500 System

Quantity1 kit

Product TypeNotebook Computer Kit

Unit SizeEach

Contents & Storage

Store at Room Temperature

Frequently asked questions (FAQs)

Can TET dye be used on the Applied Biosystems real-time PCR instruments?

TET dye is not recommended to be used on the following real-time PCR instrument models: 7000, 7300, 7500, 7500 FAST, StepOne, and StepOnePlus Systems.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What dyes can I use as reporter dyes in a multiplex reaction on the Applied Biosystems 7300 Real-Time PCR Systems?

The Applied Biosystems 7300 Real-Time PCR System has 4 signal detection filters: Filter A (FAM and SYBR Green), Filter B (VIC and JOE), Filter C (TAMRA, NED and Cy3) and Filter D (ROX and Texas Red). For multiplexing reactions, the reporter dyes from the different assays should be chosen from different filter sets, so they can be compatible. In addition, the reporter dye need to be compatible with the quencher and the passive reference dye that might be present in the reaction. The commonly used combination is FAM and VIC.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What are the thermal cycling parameters of the Fast mode for the 7500 Fast Real-Time PCR System?

For the 7500 Fast real time PCR systems, the thermal cycling parameters are defaulted to 50°C/2 min for UNG incubation, 95°C/20 sec for fast polymerase activation, 40 cycles of 95°C/3 secs for the dissociation and 60°C/30 seconds for annealing and extensions.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I run slower, standard thermal cycling experiments on a 7500 Fast Real-Time PCR System?

Yes. you can choose either standard or fast ramp on the 7500 Fast System for software versions 2x or greater, and a 9600 emulation thermal cycling mode is available for customers who wish to run assays based on adjusted ramp rate. However, you should be aware that we cannot guarantee identical performance to the same modes run on a standard 7500 system block.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I compare the data collected when using Fast thermal cycling mode compared to using standard thermal cycling mode on the 7500 Fast Real-Time PCR System?

Runs can be compared between standard and fast mode, as the data collected are collected in all five filters in the instrument. However, keep in mind that these data may not always show identical results and may have added variability due to the data collection processes and differences in ramp rate between standard and fast mode.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

View all Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems FAQs

Citations & References (3428)

Citations & References

Abstract

Authors:

Journal:

PubMed ID:11282963

Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress: Use of APE1 small molecule inhibitors to delineate APE1 functions

Authors:Jiang, YL; Guo, CL; Fishel, ML; Wang, ZY; Vasko, MR; Kelley, MR

Journal:DNA REPAIR

PubMed ID:

Oxidative DNA damage has been implicated in a number of central nervous system pathologies. The base excision repair (BER) pathway is one of the most important cellular protection mechanisms that respond to oxidative DNA damage. Human apurinic (apyrimidinic) endonuclease/redox effector factor (APE1/Ref1 or APE1) is an essential enzyme in the ... More

Characterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 and their inhibitors in equine granulosa cells in vivo and in vitro

Authors:Sessions, DR; Vick, MM; Fitzgerald, BP

Journal:JOURNAL OF ANIMAL SCIENCE

PubMed ID:

Matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMP) regulate tissue remodeling events necessary for ovulation. Thus, changes in MMP and TIMP expression and protein enzyme activity were examined in vivo and in vitro during follicular development and atresia in the horse. Equine granulosa cells and follicular fluid from medium ... More

The time of prenatal immune challenge determines the specificity of inflammation-mediated brain and behavioral pathology.

Authors:Meyer U, Nyffeler M, Engler A, Urwyler A, Schedlowski M, Knuesel I, Yee BK, Feldon J

Journal:J Neurosci

PubMed ID:16672647

Disturbance to early brain development is implicated in several neuropsychiatric disorders including autism, schizophrenia, and mental retardation. Epidemiological studies have indicated that the risk of developing these disorders is enhanced by prenatal maternal infection, presumably as a result of neurodevelopmental defects triggered by cytokine-related inflammatory events. Here, we demonstrate that ... More

Preclinical pharmacokinetic and pharmacodynamic evaluation of metronomic and conventional temozolomide dosing regimens.

Authors:Zhou Q, Guo P, Wang X, Nuthalapati S, Gallo JM

Journal:J Pharmacol Exp Ther

PubMed ID:17259446

Metronomic dosed (MD) chemotherapy as opposed to conventional dosed (CD) chemotherapy is considered an alternate strategy to target angiogenesis and limit host toxicity. Although this approach is promising, there has not been any attempt to define optimal metronomic dosing regimens by integrating pharmacokinetic (PK) with pharmacodynamic (PD) measurements. The aim ... More

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