Search
Citations & References (11)
Invitrogen™
EnzChek™ Lysozyme Assay Kit
The EnzChek™ Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. TheRead more
| Catalog Number | Quantity |
|---|---|
| E22013 | 400 Assays |
Catalog number E22013
Price (MXN)
-
Quantity:
400 Assays
The EnzChek™ Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. The increase in fluorescence resulting from lysozyme activity is measured using a fluorometer or fluorescence microplate reader.
See our complete line of Fluorescence Microplate assays.
• Detect lysozyme activity concentrations as low as 20 U/mL
• Use standard fluorescein (FITC) excitation/emission settings
• Format allows for continuous measurement of lysozyme activity
The assay utilizes an innovative approach to measure lysozyme activity. Micrococcus lysodeikticus cell walls are used that have been extensively labeled with fluorescein causing quench of the fluorescence signal. Active lysozyme enzyme hydrolyzes the b-(1-4)-glucosidic linkages between the N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the mucopolysaccharide cell wall, relieving the quenching and yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.
See our complete line of Fluorescence Microplate assays.
• Detect lysozyme activity concentrations as low as 20 U/mL
• Use standard fluorescein (FITC) excitation/emission settings
• Format allows for continuous measurement of lysozyme activity
The assay utilizes an innovative approach to measure lysozyme activity. Micrococcus lysodeikticus cell walls are used that have been extensively labeled with fluorescein causing quench of the fluorescence signal. Active lysozyme enzyme hydrolyzes the b-(1-4)-glucosidic linkages between the N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the mucopolysaccharide cell wall, relieving the quenching and yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence Intensity
Quantity400 Assays
Shipping ConditionRoom Temperature
Substrate PropertiesLipid-Based Substrate
Substrate TypeLysozyme Substrate
Target EnzymeLysozyme
For Use With (Application)Lysozyme Assay
For Use With (Equipment)Fluorescence Microplate Reader
Product LineEnzChek
Product TypeEnzChek Assay Kit
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Frequently asked questions (FAQs)
How can I limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013)?
How was the substrate (Component A) in the EnzChek Lysozyme Assay Kit labeled?
May other buffers be used for the EnzChek Lysozyme assay?
Citations & References (11)
Citations & References
Abstract
Vogesella mureinivorans sp. nov., a peptidoglycan-degrading bacterium from lake water.
Journal:Int J Syst Evol Microbiol
PubMed ID:19946047
'A novel, non-pigmented, rod-shaped, Gram-negative strain was isolated from mesotrophic lake water in Zealand, Denmark. Phylogenetic analysis of the 16S rRNA gene sequence of the bacterium, designated strain 389(T), indicated that the strain belonged to the genus Vogesella and formed a monophyletic group with Vogesella perlucida DS-28(T) (99.1?% nucleotide similarity);
Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa.
Journal:Microbiology
PubMed ID:21873404
'Bacterial pathogens use a range of protein secretion systems to colonize their host. One recent addition to this arsenal is the type VI secretion system (T6SS), which is found in many Gram-negative bacteria. The T6SS involves 12-15 components, including a ClpV-like AAA(+) ATPase. Moreover, the VgrG and Hcp components have
Mechanisms of systemic vasodilation by lysozyme-c in septic shock.
Journal:J Appl Physiol (1985)
PubMed ID:22096116
In septic shock (SS), cardiovascular collapse is caused by the release of inflammatory mediators. We previously found that lysozyme-c (Lzm-S), released from leukocytes, contributed to systemic vasodilation in a canine model of SS. We then delineated the pathway by which this occurs in a canine carotid artery organ bath preparation
Mutations of the Listeria monocytogenes peptidoglycan N-deacetylase and O-acetylase result in enhanced lysozyme sensitivity, bacteriolysis, and hyperinduction of innate immune pathways.
Journal:Infect Immun
PubMed ID:21768286
Listeria monocytogenes is a Gram-positive intracellular pathogen that is naturally resistant to lysozyme. Recently, it was shown that peptidoglycan modification by N-deacetylation or O-acetylation confers resistance to lysozyme in various Gram-positive bacteria, including L. monocytogenes. L. monocytogenes peptidoglycan is deacetylated by the action of N-acetylglucosamine deacetylase (Pgd) and acetylated by
Alcohol use disorders affect antimicrobial proteins and anti-pneumococcal activity in epithelial lining fluid obtained via bronchoalveolar lavage.
Journal:Alcohol Alcohol
PubMed ID:20729531
Our overall objective was to examine whether characteristics of epithelial lining fluid (ELF) from subjects with alcohol use disorders (AUDs) obtained via bronchoalveolar lavage (BAL) contribute to their predisposition to pneumococcal pneumonia. We sought to compare the anti-pneumococcal activity of acellular human BAL from subjects with AUDs to matched controls.