Ponasterone A

Citations & References (7)

Invitrogen™

Ponasterone A

Ponasterone A is an analog of Ecdysone, an insect steroid hormone that regulates the metamorphosis of Drosophila melanogaster.Read more

Catalog Number	Quantity
H10101	1 mg

Catalog number H10101

Price (MXN)

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Ponasterone A is an analog of Ecdysone, an insect steroid hormone that regulates the metamorphosis of Drosophila melanogaster.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

FormatPowder

Product TypePonasterone A

Quantity1 mg

Unit SizeEach

Contents & Storage

Ponasterone A is provided as a lyophilized powder. Store at -20°C. Ponasterone A is guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

Do you still offer the Ecdysone Expression System?

The Ecdysone vectors and cell lines have been discontinued, but we do still offer the inducers, Muristerone A and Ponasterone A.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the half-life of Ponasterone A in culture medium at 37 degrees C? Is it necessary to replenish the Ponasterone A in cell culture with a long induction phase?

We do not have an exact measurement for the half-life of Ponasterone A in media. However, in studies measuring the “decay period” of expression when inducing with Ponasterone A, peak activity was seen at 24 hours post induction. There was a 30% decrease in activity at 48 hours post induction and a 70% decrease after 72 hours. Based on this data, we recommend replenishment of medium every 2-3 days with fresh inducer, which will usually correspond to cell splitting times.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can Ponasterone A be resuspended in ethanol at concentrations higher than 1 mM and can it be dissolved in other solvents such as DMSO?

Ponasterone A solution may be prepared as 5 mM stock in ethanol (250 µg of Ponasterone A in 100µL of ethanol). They will take a while to go into solution. Add ethanol and incubate at 37 degrees C for about 15 minutes, then vortex vigorously. Ponasterone A is also highly soluble in DMSO.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the molecular weight of Ponasterone A?

The molecular weight of Ponasterone A is 464.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I sequenced one of your vectors after PCR amplification and observed a difference from what is provided online (or in the manual). Should I be concerned?

Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.

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Citations & References (7)

Citations & References

Abstract

Human mitochondrial 5'-deoxyribonucleotidase. Overproduction in cultured cells and functional aspects.

Authors: Gallinaro Lisa; Crovatto Katia; Rampazzo Chiara; Pontarin Giovanna; Ferraro Paola; Milanesi Eva; Reichard Peter; Bianchi Vera;

Journal:J Biol Chem

PubMed ID:12124385

'Deoxynucleoside triphosphates (dNTPs) used for mitochondrial DNA replication are mainly formed by phosphorylation of deoxynucleosides imported into mitochondria from the cytosol. We earlier obtained evidence for a mitochondrial 5''-nucleotidase (dNT2) with a pronounced specificity for dUMP and dTMP and suggested that the enzyme protects mitochondrial DNA replication from excess dTTP. ... More

Multiple lysine mutations in the C-terminal domain of p53 interfere with MDM2-dependent protein degradation and ubiquitination

Authors:Nakamura S, Roth JA, Mukhopadhyay T

Journal:Mol Cell Biol

PubMed ID:11094089

'To investigate the effect of mutations in the p53 C-terminal domain on MDM2-mediated degradation, we introduced single and multiple point mutations into a human p53 cDNA at four putative acetylation sites (amino acid residues 372, 373, 381, and 382). Substitution of all four lysine residues by alanines (the A4 mutant) ... More

Ca2+/calmodulin-dependent protein kinase II is required for microcystin-induced apoptosis.

Authors: Fladmark Kari E; Brustugun Odd T; Mellgren Gunnar; Krakstad Camilla; Boe Roald; Vintermyr Olav K; Schulman Howard; Doskeland Stein O;

Journal:J Biol Chem

PubMed ID:11713251

'The potent natural toxins microcystin, nodularin, and okadaic acid act rapidly to induce apoptotic cell death. Here we show that the apoptosis correlates with protein phosphorylation events and can be blocked by protein kinase inhibitors directed against the multifunctional Ca(2+)/calmodulin-dependent protein kinase II (CaMKII). The inhibitors used comprised a battery ... More

Molecular basis of the specific subcellular localization of the C2-like domain of 5-lipoxygenase.

Authors: Kulkarni Shilpa; Das Sudipto; Funk Colin D; Murray Diana; Cho Wonhwa;

Journal:J Biol Chem

PubMed ID:11796736

The activation of 5-lipoxygenase (5-LO) involves its calcium-dependent translocation to the nuclear envelope, where it catalyzes the two-step transformation of arachidonic acid into leukotriene A(4), leading to the synthesis of various leukotrienes. To understand the mechanism by which 5-LO is specifically targeted to the nuclear envelope, we studied the membrane ... More

New Human Breast Cancer Cells to Study Progesterone Receptor Isoform Ratio Effects and Ligand-independent Gene Regulation.

Authors: Jacobsen Britta M; Richer Jennifer K; Schittone Stephanie A; Horwitz Kathryn B;

Journal:J Biol Chem

PubMed ID:12021276

All known progesterone target cells coexpress two functionally different progesterone receptor (PR) isoforms: 120-kDa B-receptors (PR-B) and N-terminally truncated, 94-kDa A-receptors (PR-A). Their ratio varies in normal and malignant tissues. In human breast cancer cells, homodimers of progesterone-occupied PR-A or PR-B regulate different gene subsets. To study PR homo- and ... More

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