Ponasterone A

Do you still offer the Ecdysone Expression System?

The Ecdysone vectors and cell lines have been discontinued, but we do still offer the inducers, Muristerone A and Ponasterone A.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the half-life of Ponasterone A in culture medium at 37 degrees C? Is it necessary to replenish the Ponasterone A in cell culture with a long induction phase?

We do not have an exact measurement for the half-life of Ponasterone A in media. However, in studies measuring the “decay period” of expression when inducing with Ponasterone A, peak activity was seen at 24 hours post induction. There was a 30% decrease in activity at 48 hours post induction and a 70% decrease after 72 hours. Based on this data, we recommend replenishment of medium every 2-3 days with fresh inducer, which will usually correspond to cell splitting times.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can Ponasterone A be resuspended in ethanol at concentrations higher than 1 mM and can it be dissolved in other solvents such as DMSO?

Ponasterone A solution may be prepared as 5 mM stock in ethanol (250 µg of Ponasterone A in 100µL of ethanol). They will take a while to go into solution. Add ethanol and incubate at 37 degrees C for about 15 minutes, then vortex vigorously. Ponasterone A is also highly soluble in DMSO.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the molecular weight of Ponasterone A?

The molecular weight of Ponasterone A is 464.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I sequenced one of your vectors after PCR amplification and observed a difference from what is provided online (or in the manual). Should I be concerned?

Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.

Are your vectors routinely sequenced?

No, our vectors are not routinely sequenced. Quality control and release criteria utilize other methods.

How was the reference sequence for your vectors created?

Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources.

Ponasterone A - FAQs