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Invitrogen
BRCA1 Monoclonal Antibody (MS110)
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FIGURE: 1 / 3
BRCA1 Antibody (MA1-137) in IHC (P)
Immunohistochemistry analysis of BRCA1 was performed on human breast carcinoma tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 8-15 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide-methanol for 15 min at room temperature. Tissue slides were washed with deionized water and P... View More
Product Details
MA1-137
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MS110
Immunogen
Recombinant full-length human BRCA1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody binding site (i.e.epitope) has been mapped within the N-terminal (1-304 amino acids) of Human BRCA1.
Target Information
BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: brca 1; brca-1; BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; breast cancer 1 early onset; breast cancer 1, early onset; Breast cancer type 1 susceptibility protein; breast cancer type 1 susceptibility protein isoform p.23; breast cancer type 1 susceptibility protein, isoform p.10; breast cancer type 1 susceptibility protein, isoform p.11; breast cancer type 1 susceptibility protein, isoform p.12; breast cancer type 1 susceptibility protein, isoform p.13; breast cancer type 1 susceptibility protein, isoform p.14; breast cancer type 1 susceptibility protein, isoform p.15; breast cancer type 1 susceptibility protein, isoform p.16; breast cancer type 1 susceptibility protein, isoform p.17; breast cancer type 1 susceptibility protein, isoform p.18; breast cancer type 1 susceptibility protein, isoform p.19; breast cancer type 1 susceptibility protein, isoform p.20; breast cancer type 1 susceptibility protein, isoform p.21; breast cancer type 1 susceptibility protein, isoform p.22; breast cancer type 1 susceptibility protein, isoform p.24; breast cancer type 1 susceptibility protein, isoform p.25; breast cancer type 1 susceptibility protein, isoform p.26; breast cancer type 1 susceptibility protein, isoform p.27; breast cancer type 1 susceptibility protein, isoform p.28; breast cancer type 1 susceptibility protein, isoform p.29; breast cancer type 1 susceptibility protein, isoform p.30; breast cancer type 1 susceptibility protein, isoform p.31; breast cancer type 1 susceptibility protein, isoform p.32; breast cancer type 1 susceptibility protein, isoform p.33; breast cancer type 1 susceptibility protein, isoform p.34; breast cancer type 1 susceptibility protein, isoform p.35; breast cancer type 1 susceptibility protein, isoform p.36; breast cancer type 1 susceptibility protein, isoform p.37; breast cancer type 1 susceptibility protein, isoform p.38; breast cancer type 1 susceptibility protein, isoform p.39; breast cancer type 1 susceptibility protein, isoform p.40; breast cancer type 1 susceptibility protein, isoform p.41; breast cancer type 1 susceptibility protein, isoform p.42; breast cancer type 1 susceptibility protein, isoform p.6; breast cancer type 1 susceptibility protein, isoform p.7; breast cancer type 1 susceptibility protein, isoform p.8; breast cancer type 1 susceptibility protein, isoform p.9; BROVCA12; Delta772-3095; early onset breast cancer 1; Fanconi anemia, complementation group S; influences susceptibility to breast and ovarian cancer; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; protein phosphatase 1, regulatory subunit 53; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; unnamed protein product
Gene Aliases: BRCA1; BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
UniProt ID: (Human) P38398
Entrez Gene ID: (Human) 672
transcription regulatory region sequence-specific DNA binding
p53 binding
DNA binding
damaged DNA binding
transcription coactivator activity
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
tubulin binding
transferase activity
enzyme binding
ubiquitin protein ligase binding
identical protein binding
metal ion binding
ubiquitin protein ligase activity
ubiquitinated histone binding
RNA polymerase binding
histone H2AK127 ubiquitin ligase activity
histone H2AK129 ubiquitin ligase activity
double-strand break repair via homologous recombination
DNA repair
regulation of DNA repair
postreplication repair
double-strand break repair
DNA recombination
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
lipid metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
cellular response to DNA damage stimulus
chromosome segregation
mitotic G2 DNA damage checkpoint
centrosome cycle
dosage compensation
intrinsic apoptotic signaling pathway in response to DNA damage
response to ionizing radiation
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
protein ubiquitination
negative regulation of cell growth
negative regulation of intracellular estrogen receptor signaling pathway
reciprocal DNA recombination
chordate embryonic development
hypermethylation of CpG island
mitotic G2/M transition checkpoint
negative regulation of fatty acid biosynthetic process
positive regulation of DNA repair
positive regulation of angiogenesis
negative regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of centriole replication
regulation of cell cycle
protein autoubiquitination
random inactivation of X chromosome
cellular response to tumor necrosis factor
cellular response to ionizing radiation
cellular response to indole-3-methanol
protein K6-linked ubiquitination
DNA strand resection involved in replication fork processing
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of DNA damage checkpoint
negative regulation of reactive oxygen species metabolic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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