Search
Invitrogen
PDK4 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 8
PDK4 Antibody (PA5-13776) in ICC/IF
Immunocytochemistry analysis of PDK4 in HeLa cells. Samples were incubated with PDK4 polyclonal antibody (Product # PA5-13776) using a dilution of 1:100 for 2 h at room temperature followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:1,000 for 1hr. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.2%, 30 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.
Product Details
PA5-13776
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
400 µg
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
PDK4 (PDHK4), along with the other PDHKs, regulates glucose metabolism by phosphorylating the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex. Inhibition of PDHKs is viewed as a potential therapeutic treatment for type II diabetes. This protein is located in the matrix of the mitrochondria and it's expression is regulated by glucocorticoids, retinoic acid and insulin. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; kinase isozyme 4; lipoamide; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 4; Pyruvate dehydrogenase kinase isoform 4; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial; unnamed protein product
Gene Aliases: AV005916; PDHK4; PDK4
UniProt ID: (Human) Q16654, (Mouse) O70571
Entrez Gene ID: (Human) 5166, (Mouse) 27273
regulation of pH
insulin receptor signaling pathway
cellular response to starvation
regulation of acetyl-CoA biosynthetic process from pyruvate
regulation of cellular ketone metabolic process
regulation of glucose metabolic process
cellular response to stress
regulation of fatty acid biosynthetic process
glucose homeostasis
response to starvation
regulation of bone resorption
regulation of fatty acid oxidation
cellular response to fatty acid
reactive oxygen species metabolic process
negative regulation of anoikis
positive regulation of defense response to virus by host
carbohydrate metabolic process
glucose metabolic process
acetyl-CoA biosynthetic process from pyruvate
protein phosphorylation
phosphorylation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support