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Invitrogen
Phospho-MEK3 (Thr222) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-MEK3 (Thr222) Antibody (PA5-37699) in IHC (P)
Immunohistochemical analysis of Phospho-MEK3 (Thr222) in paraffin-embedded Human brain tissue using Phospho-MEK3 (Thr222) Polyclonal Antibody (Product # PA5-37699) (left) or the same antibody preincubated with blocking peptide (right).
Product Details
PA5-37699
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide sequence around phosphorylation site of threonine 222(A-K-T(p)-M-D) derived from Human MAP2K3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control for Western blot is Jurkat cells; suggested positive control for IHC is human brain tissue.
Target Information
MAP2K3 (MEK3) is a dual-specificity protein kinase which acts in cellular signal transduction pathways, and is necessary for the expression of glucose transporter. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for MEK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 3; EC 2.7.12.2; MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK 3; SAPK kinase 2; Stress-activated protein kinase kinase 2; unnamed protein product
Gene Aliases: MAP2K3; MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2; SKK2
UniProt ID: (Human) P46734
Entrez Gene ID: (Human) 5606
MAPK cascade
regulation of cytokine production
response to ischemia
inflammatory response
signal transduction
heart development
stress-activated protein kinase signaling cascade
negative regulation of hippo signaling
cellular response to vascular endothelial growth factor stimulus
p38MAPK cascade
positive regulation of MAPK cascade
positive regulation of blood vessel endothelial cell migration
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
protein maturation
cardiac muscle contraction
pyroptosis
cellular response to lipopolysaccharide
cellular response to UV-B
cellular response to sorbitol
cellular senescence
regulation of intracellular signal transduction
NLRP1 inflammasome complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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