Achieve single-stranded DNA quantification using the Qubit ssDNA Assay Kit with any fluorometer or plate reader. Simply dilute the reagent using the buffer provided, add your sample, and read the concentration using a Qubit or other fluorometer. The assay detects oligonucleotides or long ssDNA, and common contaminants such as salts, solvents, detergents, and protein are well tolerated. Nucleotides and short oligonucleotides of six bases or fewer do not interfere in this quantitation assay.

The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but can be used with any fluorometer or fluorescence plate reader. The Qubit ssDNA kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA, but it will not detect contaminating protein or nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg/μL to 200 ng/μL, enabling an assay range of 1–200 ng.

The kit includes concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, and protein are well tolerated in this assay.

Which product do you choose for ssDNA quantitation?
• 1–20 samples: use this Qubit ssDNA Assay Kit with the Qubit Fluorometer
• 20–2000 samples: use the Quant-iT OliGreen ssDNA Assay Kit with a microplate reader

Notes:
1. The Qubit ssDNA Assay Kit can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 μL thin-walled PCR tubes (Cat. No. Q32856) are required but not included.