MDY-64 is a green-fluorescent yeast vacuole membrane stain.Read more
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Catalog Number
Quantity
Y7536
1 mg
Catalog number Y7536
Price (MXN)
-
Quantity:
1 mg
MDY-64 is a green-fluorescent yeast vacuole membrane stain.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Quantity1 mg
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeMembrane Stain
SubCellular LocalizationVacuoles
Unit Size1 mg
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Citations & References (10)
Citations & References
Abstract
Effect of the substitution of muscle actin-specific subdomain 1 and 2 residues in yeast actin on actin function.
Authors:McKane M, Wen KK, Meyer A, Rubenstein PA
Journal:J Biol Chem
PubMed ID:16882670
'Muscle and yeast actins display distinct behavioral characteristics. To better understand the allosteric interactions that regulate actin function, we created a muscle/yeast hybrid actin containing a muscle-specific outer domain (subdomains 1 and 2) and a yeast inner domain (subdomains 3 and 4). Actin with muscle subdomain 1 and the two ... More
Structure, function, and motility of vacuoles in filamentous fungi
Authors:Cole L, Orlovich DA, Ashford AE
Journal:Fungal Genet Biol
PubMed ID:9742195
'Current information on the structure and function of motile tubular vacuoles in Pisolithus tinctorius and other fungi is reviewed. The use of fluorochromes to label the vacuole lumen is evaluated and observations on the structure and motility of vacuoles in P. tinctorius are differentiated from possible artifacts. The styryl dyes ... More
Interdependent assembly of specific regulatory lipids and membrane fusion proteins into the vertex ring domain of docked vacuoles.
Authors:Fratti RA, Jun Y, Merz AJ, Margolis N, Wickner W
Journal:J Cell Biol
PubMed ID:15611334
'Membrane microdomains are assembled by lipid partitioning (e.g., rafts) or by protein-protein interactions (e.g., coated vesicles). During docking, yeast vacuoles assemble "vertex" ring-shaped microdomains around the periphery of their apposed membranes. Vertices are selectively enriched in the Rab GTPase Ypt7p, the homotypic fusion and vacuole protein sorting complex (HOPS)-VpsC Rab ... More
Physiological regulation of yeast cell death in multicellular colonies is triggered by ammonia.
Authors:Váchová L, Palková Z
Journal:J Cell Biol
PubMed ID:15939758
The existence of programmed cell death (PCD) in yeast and its significance to simple unicellular organisms is still questioned. However, such doubts usually do not reflect the fact that microorganisms in nature exist predominantly within structured, multicellular communities capable of differentiation, in which a profit of individual cells is subordinated ... More
Remodeling of organelle-bound actin is required for yeast vacuole fusion.
Authors:Eitzen G, Wang L, Thorngren N, Wickner W
Journal:J Cell Biol
PubMed ID:12177043
Actin participates in several intracellular trafficking pathways. We now find that actin, bound to the surface of purified yeast vacuoles in the absence of cytosol or cytoskeleton, regulates the last compartment mixing stage of homotypic vacuole fusion. The Cdc42p GTPase is known to be required for vacuole fusion. We now ... More