Anfotericina B
Anfotericina B
Citas y referencias (10)
Gibco™

Anfotericina B

La anfotericina B es el principio activo de Fungizone. Fungizone es una marca comercial de E.R. Squibb & Sons, LLC.Más información
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Número de catálogoCantidad
1529002650 mL
1529001820 ml
Número de catálogo 15290026
Precio (MXN)
2,218.81
Each
Añadir al carro de la compra
Cantidad:
50 mL
Precio (MXN)
2,218.81
Each
Añadir al carro de la compra
La anfotericina B es el principio activo de Fungizone. Fungizone es una marca comercial de E.R. Squibb & Sons, LLC. La anfotericina B es un antifúngico producido por Streptomyces nodosus. Previene el crecimiento de hongos al provocar un aumento de la permeabilidad de la membrana plasmática fúngica. Se une activamente a los esteroles y da lugar a la formación de poros. Se usa para prevenir la contaminación de cultivos de células con levaduras y hongos multicelulares. La anfotericina B Gibco contiene 250 µg de anfotericina B y 205 µg de deoxicolato sódico por ml de agua destilada. La concentración de trabajo recomendada oscila entre 0,25 y 2,50 µg/ml.

Fabricación según las buenas prácticas de fabricación actuales en dos instalaciones
Para mantener la continuidad de la cadena de suministro, fabricamos la anfotericina B Gibco en dos instalaciones, una ubicada en Grand Island, NY (EE. UU.) y la otra en Escocia (Reino Unido). Ambos sitios cumplen los requisitos de producción según las buenas prácticas de fabricación actuales, cuentan con la certificación ISO 13485 estándar y están registrados en la FDA como fabricantes de dispositivos médicos.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
ConcentraciónDe 0,25 a 2,5 μg/ml
Para utilizar con (aplicación)Prevención de la contaminación del cultivo celular
Línea de productosFungizone
Cantidad50 mL
Duración de almacenamiento12 meses
Condiciones de envíoHielo seco
FormularioLíquido
Tipo de productoAntifúngico
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De – 5 a – 20 °C
Condiciones de envío: Congelado en hielo seco
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

How many freeze/thaw cycles do you recommend for Amphotericin B?

Amphotericin B can be freeze-thawed 2-3 times without appreciable loss of potency.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the stability of Amphotericin B when stored at 4 degrees C?

Amphotericin B in solution is stable at 2-8 degrees C for approximately 4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Fungizone and Amphotericin B?

Amphotericin B is the generic version of Fungizone. Fungizone is a trademark of E.R. Squibb & Sons, LLC.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (10)

Citations & References
Abstract
Generation of human brain region-specific organoids using a miniaturized spinning bioreactor.
Authors:Qian X, Jacob F, Song MM, Nguyen HN, Song H, Ming GL
Journal:Nat Protoc
PubMed ID:29470464
Human brain organoids, 3D self-assembled neural tissues derived from pluripotent stem cells, are important tools for studying human brain development and related disorders. Suspension cultures maintained by spinning bioreactors allow for the growth of large organoids despite the lack of vasculature, but commercially available spinning bioreactors are bulky in size ... More
PC12 Cell Line: Cell Types, Coating of Culture Vessels, Differentiation and Other Culture Conditions.
Authors:Wiatrak B, Kubis-Kubiak A, Piwowar A, Barg E
Journal:Cells
PubMed ID:32295099
'The PC12 cell line is one of the most commonly used in neuroscience research, including studies on neurotoxicity, neuroprotection, neurosecretion, neuroinflammation, and synaptogenesis. Two types of this line are available in the ATCC collection: traditional PC12 cells grown in suspension and well-attached adherent phenotype. PC12 cells grown in suspension tend ... More
Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides.
Authors:Arrigo AP
Journal:Methods Mol Biol
PubMed ID:29177658
Human HspB1 (also denoted as Hsp27) belongs to the family of small (or stress) proteins (sHsps). The family, which contains ten members including aA,B-crystallin polypeptides, is characterized by a conserved C-terminal a-crystallin domain and molecular weights ranging from 20 to 40 kDa. Here, procedures are described for analyzing the dynamic oligomerization ... More
A protective Zika virus E-dimer-based subunit vaccine engineered to abrogate antibody-dependent enhancement of dengue infection.
Authors:Slon-Campos JL, Dejnirattisai W, Jagger BW, López-Camacho C, Wongwiwat W, Durnell LA, Winkler ES, Chen RE, Reyes-Sandoval A, Rey FA, Diamond MS, Mongkolsapaya J, Screaton GR
Journal:Nat Immunol
PubMed ID:31477918
Infections with dengue virus (DENV) and Zika virus (ZIKV) can induce cross-reactive antibody responses. Two immunodominant epitopes-one to precursor membrane protein and one to the fusion loop epitope on envelope (E) protein-are recognized by cross-reactive antibodies ... More
Establishment and Genetic Landscape of Precancer Cell Model Systems from the Head and Neck Mucosal Lining.
Authors:de Boer DV, Brink A, Buijze M, Stigter-van Walsum M, Hunter KD, Ylstra B, Bloemena E, Leemans CR, Brakenhoff RH
Journal:Mol Cancer Res
PubMed ID:30224542
Head and neck squamous cell carcinomas (HNSCC) develop in fields of genetically altered cells. These fields are often dysplastic and a subset can be recognized as (erythro)leukoplakia, but most are macroscopically invisible. There is a lack of adequate treatment options to eradicate these fields, whereas they underlie the development of ... More
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