PDPH (3-(2-pyridyldithio)propionyl hydrazide)
PDPH (3-(2-pyridyldithio)propionyl hydrazide)
Citas y referencias (3)
Thermo Scientific™

PDPH (3-(2-pyridyldithio)propionyl hydrazide)

Thermo Scientific Pierce PDPH es un entrecruzador corto de piridilditiol e hidracida para conjugar sulfhidrilos (cisteínas) a carbonilos (aldehído oMás información
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Número de catálogoCantidad
2230150 mg
Número de catálogo 22301
Precio (MXN)
-
Cantidad:
50 mg
Thermo Scientific Pierce PDPH es un entrecruzador corto de piridilditiol e hidracida para conjugar sulfhidrilos (cisteínas) a carbonilos (aldehído o cetonas, como las formadas por oxidación de carbohidratos de glicoproteínas).

Características de PDPH:

• Grupos reactivos: Piridil disulfuro e hidracida
• Reactivos hacia: grupos de sulfhidrilo y carbonilo (aldehído)
• Entrecruzador corto (9,2A) de sulfhidrilo a aldehído con brazo espaciador de enlaces disulfuro (escindible)
• El grupo piridilditiol resulta en la adhesión a sulfhidrilos a través de enlace disulfuro, que puede escindirse con DTT, TCEP u otros agentes reductores
• El grupo de hidracida conjuga a azúcares oxidados de glicoproteínas y carbohidratos
• Utilizar metaperiodato sódico para oxidar la glicosilación (por ejemplo, ácido siálico) a grupos de aldehído reactivos
• Utilizar con EDC para conjugar la amina primaria del grupo de hidracida a grupos carboxilo

Referencias de producto:
Guía de aplicación entrecruzada: búsqueda de referencias literarias recientes para este producto
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularSí
DescripciónPDPH
FormularioPolvo
Método de etiquetadoEtiqueta química
Peso molecular229.32
PegiladoNo
Línea de productosPierce
Cantidad50 mg
Fracción reactivaHidrazida, piridildisulfuro
Condiciones de envíoAmbiente
SolubilidadDMF, DMSO
Longitud del brazo del separador9,2 Å
Soluble en aguaNo
Reactividad químicaCarbohidrato-Sulfhidrilo
CleavablePor tioles
Tipo de enlace cruzadoHeterobifuncionales
FormatoEstándar
Tipo de productoEntrecruzador
SeparadorCorto (< 10 Å)
Unit SizeEach
Contenido y almacenamiento
Tras su recepción, almacenar desecado a 4 °C.

Preguntas frecuentes

Can you provide the shelf-life for PDPH (3-(2-pyridyldithio)propionyl hydrazide)?

PDPH (3-(2-pyridyldithio)propionyl hydrazide) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

We are using EDC to crosslink a protein onto carboxyl-modified beads. We would like to add in a linker molecule to extend the radius of beads to increase the surface area so more proteins can be immobilized. Is this possible?

To provide a linker between the beads and the target protein, you would need a molecule with a primary amine (to conjugate to the carboxyl group on the beads with EDC) on one end and another reactive group on the other (to conjugate to the protein). For instance, sulfo-SMCC (Cat. No. A39268) could be used in conjunction with PDPH (Cat. No. 22301):
The method is outlined below:
1. Conjugate the target protein to the NHS ester (amine-reactive) group of Sulfo-SMCC. This will produce a maleimide-activated target protein via its primary amines (lysines and N-terminus).
2. Remove the excess sulfo-SMCC by desalting or dialysis.
3. React the maleimide-activated target protein to PDPH via sulfhydryls. (Lysines and N-terminus now are connected to hydrazide (amine) group with an extended linker)
4. Remove excess PDPH by desalting or dialysis.
5. React the hydrazide-activated target protein with carboxyl beads in the presence of EDC.
6. Use appropriate buffer to rinse off excess target protein from the carboxyl beads.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (3)

Citations & References
Abstract
Systemic administration of a nerve growth factor conjugate reverses age-related cognitive dysfunction and prevents cholinergic neuron atrophy.
Authors:Bäckman C, Rose GM, Hoffer BJ, Henry MA, Bartus RT, Friden P, Granholm AC
Journal:J Neurosci
PubMed ID:8757256
'Intraventricular administration of nerve growth factor (NGF) in rats has been shown to reduce age-related atrophy of central cholinergic neurons and the accompanying memory impairment. Intraventricular administration of NGF is necessary because NGF will not cross the blood-brain barrier (BBB). Here we have used a novel carrier system, consisting of ... More
Conjugation of folate via gelonin carbohydrate residues retains ribosomal-inactivating properties of the toxin and permits targeting to folate receptor positive cells.
Authors:Atkinson SF, Bettinger T, Seymour LW, Behr JP, Ward CM
Journal:J Biol Chem
PubMed ID:11359781
Conjugation of folate to proteins permits receptor-mediated endocytosis via the folate receptor (FR) and delivery of the conjugate into the cytoplasm of cells. Since many cancers up-regulate the FR it has enabled the targeting of toxins to tumor cells resulting in specific cell death. However, current conjugation methods rely on ... More
Site-specific modifications of light chain glycosylated antilymphoma (LL2) and anti-carcinoembryonic antigen (hImmu-14-N) antibody divalent f1agments.
Authors:Govindan SV, Goldenberg DM, Griffiths GL, Leung SO, Losman MJ, Hansen HJ
Journal:Cancer Res
PubMed ID:7493334
Site-specific introduction of metal-chelating groups into F(ab')2 fragments of an antilymphoma antibody (LL2) possessing a natural Asn-linked light chain carbohydrate and an anti-carcinoembryonic antigen antibody (hImmu-14-N) grafted with a light chain carbohydrate site is described. For this purpose, four yttrium- (and indium)-chelating agents were used, containing a primary amino group ... More