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Invitrogen
MUC5B Monoclonal Antibody (5B19-2E)
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FIGURE: 1 / 4
MUC5B Antibody (37-7400) in ICC/IF
Immunofluorescence analysis of MUC5B was performed using 70 % confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUC5B (5B19-2E) Mouse Monoclonal antibody (Product # 37-7400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
Product Details
37-7400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
5B19-2E
Immunogen
Synthetic peptide derived from the N-terminal region of the human MUC5B protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Both IHC and WB applications require the following deglycosylation protocols.
Immunohistochemistry
1. Prepare the following stock solutions:
a. 200 mM NaIO4 (8.556 g/200 mL water)
b. Sodium-Acetate buffer, pH 4.5: Dissolve NaCl (final concentration: 0.33M) into 0.1M glacial acetic acid and adjust to pH 4.5 using 1 N NaOH.
c. Sodium-thiosulfate solution: Dissolve Na2S2O3 (0.133M), NaI (0.033M), and NaHCO3 (0.033M) in water and adjust to pH 7.6 using 1M HCl. Keep solution at 4°C.
2. After deparaffinization, rehydration, and peroxidase quenching steps, mix a 1:1 solution of 'a' and 'b' (in step 1), add to each slide, and incubate overnight at 4°C.
3. Treat tissue with solution 'c' for 30 minutes at 4°C.
4. Proceed with normal immunostaining protocol (blocking and antibody incubation steps).
Western blotting
1. Treat transblotted membrane with 10 mM NaIO4 in 0.1M sodium acetate/5 mM EDTA, pH 5.5 overnight at 4°C in the dark.
2. After washing off the solution, incubate the membrane in a solution containing 30 mM Na2S2O3, 7.5 mM NaI, and 7.5 mM NaHCO3, pH 7.2 for 1 hour at 4°C in the dark.
3. Proceed with normal Western blot protocol (wash and blocking steps).
Target Information
Mucins (MUCs) are heavily glycosylated glycoproteins that are synthesized either as membrane-bound or secreted proteins. Mucins are noteworthy because they have an aberrant expression profile in various malignancies. Mucins normally play a protective role for epithelial tissues and are expressed in the gastrointestinal, respiratory, and genito-urinary tracts. Mucins may also be involved in the renewal and differentiation of the epithelium, and the modulation of cell adhesion and cell signaling. MUC5B is thought to play a vital role in maintaining oral health, and may also be involved in respiratory and oral disease.2 MUC5B expression has been studied in gastric carcinoma as well as chronic obstructive pulmonary disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cervical mucin; cervical mucin MUC5B; high molecular weight mucin; High molecular weight salivary mucin MG1; MUC-5B; MUC5B; mucin 5, subtype B, tracheobronchial; mucin MG1; Mucin-5 subtype B, tracheobronchial; Mucin-5B; Sublingual gland mucin; unnamed protein product
Gene Aliases: MG1; MUC-5B; MUC5; MUC5B; MUC9
UniProt ID: (Human) Q9HC84
Entrez Gene ID: (Human) 727897
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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