Insulina-transferrina-selenio (ITS-G) (100X)
Insulina-transferrina-selenio (ITS-G) (100X)
Insulina-transferrina-selenio (ITS-G) (100X)
Insulina-transferrina-selenio (ITS-G) (100X)
Citas y referencias (4)
Gibco™

Insulina-transferrina-selenio (ITS-G) (100X)

La insulina-transferrina-selenio Gibco™ (ITS-G) se utiliza como suplemento de medio basal a fin de reducir la cantidad de suero fetalMás información
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Número de catálogoCantidad
4140004510 mL
Número de catálogo 41400045
Precio (MXN)
-
Cantidad:
10 mL
La insulina-transferrina-selenio Gibco™ (ITS-G) se utiliza como suplemento de medio basal a fin de reducir la cantidad de suero fetal bovino (SFB) necesaria para cultivos celulares. La solución de ITS-G, empleada originalmente con RPMI 1640 y medio esencial mínimo (MEM), se usa ahora rutinariamente con otro medio basal para admitir una amplia variedad de tipos celulares en presencia de entre un 2 y un 4 % de SFB.

La insulina promueve la absorción de la glucosa y los aminoácidos, la lipogénesis, el transporte intracelular y la síntesis de proteínas y ácidos nucleicos. La transferrina es un portador de hierro y también puede ayudar a reducir los niveles tóxicos de radicales de oxígeno y peróxido. El selenio, como selenito sódico, es un cofactor para la glutatión peroxidasa y otras proteínas, y se utiliza como un antioxidante en el medio.

Está disponible la formulación completa.

Instalaciones que cumplen los principios de prácticas recomendadas de fabricación actuales (cGMP)
Gibco™ ITS-G se fabrica en unas instalaciones que cumplen con las prácticas correctas de fabricación actuales ubicadas en Grand Island, Nueva York (EE. UU). Las instalaciones se han registrado en la Agencia estadounidense de alimentos y medicamentos (FDA) como fabricante de dispositivos médicos y tienen la certificación según la norma ISO 13485.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaCélulas de mamíferos
Concentración100 X
Cantidad10 mL
Duración de almacenamiento18 meses
Condiciones de envíoTemperatura ambiente
FormularioLíquido
Tipo de productoTransferrina
EsterilidadEstéril con filtro
Sin aditivosSin rojo fenol
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 °C a 8 °C
Condiciones de envío: Temperatura ambiente
Vida útil: 18 meses a partir de la fecha de fabricación

Preguntas frecuentes

What is the buffer composition of the various Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

All ITS and ITS Select supplements are formulated in Earle's Balanced Salt Solution (EBSS).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

ITS Select supplements are animal origin-free (AOF) versions of the ITS supplements offered by Thermo Fisher Scientific.

ITS Select supplements use recombinant versions of both proteins in the formulation: insulin and transferrin.

All other versions of ITS, those without the Select designation, utilize recombinant insulin and a human-derived holo transferrin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the individual concentrations of insulin, transferrin, and selenium in the Insulin-Transferrin-Selenium (ITS) (100X) supplements?

All ITS and ITS Select supplements have the following concentrations of their components:

Insulin: 1.0 g/L
Transferrin: 0.55 g/L
Selenium: 0.00067 g/L

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store aliquots of Insulin-Transferrin-Selenium (ITS) (100X) supplements at -20 degrees C without compromising the integrity of the product?

We recommend storing all ITS and ITS Select supplements at 2-8 degrees C as there is a chance that the insulin will precipitate upon freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are all Insulin-Transferrin-Selenium (ITS) (100X) supplements offered by Thermo Fisher Scientific animal origin-free?

We offer both animal origin and animal origin-free versions of our ITS supplements. The animal origin-free product versions are referred to as ITS Select supplements.

Insulin-Transferrin-Selenium (ITS-G) (100X) (Cat. No. 41400045), Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (100X) (Cat. No. 51500056), and Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044) are not animal origin-free.

Animal origin-free product versions are available for the ITS-G and ITS-X formulations: Insulin-Transferrin-Selenium (ITS-G) Select Supplement (100X) (Cat. No. A4000046501 and A4000046502) and Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) Select Supplement (100X) (Cat. No. A4000046401 and Cat. No. A4000046402.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Insulina-transferrina-selenio (ITS-G) (100X) FAQs

Citations & References (4)

Citations & References
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce ... More
Collective cell migration requires suppression of actomyosin at cell-cell contacts mediated by DDR1 and the cell polarity regulators Par3 and Par6.
Authors:Hidalgo-Carcedo C, Hooper S, Chaudhry SI, Williamson P, Harrington K, Leitinger B, Sahai E,
Journal:Nat Cell Biol
PubMed ID:21170030
Collective cell migration occurs in a range of contexts: cancer cells frequently invade in cohorts while retaining cell-cell junctions. Here we show that collective invasion by cancer cells depends on decreasing actomyosin contractility at sites of cell-cell contact. When actomyosin is not downregulated at cell-cell contacts, migrating cells lose cohesion. ... More
Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology.
Authors:Smits AM, van Vliet P, Metz CH, Korfage T, Sluijter JP, Doevendans PA, Goumans MJ,
Journal:Nat Protoc
PubMed ID:19197267
To date, there is no suitable in vitro model to study human adult cardiac cell biology. Although embryonic stem cells are able to differentiate into cardiomyocytes in vitro, the efficiency of this process is very low. Other methods to differentiate progenitor cells into beating cardiomyocytes rely on coculturing with rat ... More