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Rockland
Phospho-CDC27 (Thr244) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-CDC27 (Thr244) Antibody (600-401-885) in WB
Western blot using Rocklands Affinity Purified anti-CDC27 pT244 antibody shows detection of a band ~92 kDa corresponding to phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Phosphorylated CDC27 is mostly present only in cell preparations arrested in mitosis. Each lane contains approximately 30 µg of HeLa whole cell ... View More
Product Details
600-401-885
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion surrounding T244 of Human CDC27.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against the phosphorylated form of human CDC27 at the pT244 residue. Reactivity occurs against human CDC27 pT244 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CDC27 is minimal by ELISA. The antibody does not cross-react with CDC27 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CDC27 protein from mouse, rat and chicken may occur as sequence homology varies by one amino acid residue in this sequence (90% homology). Cross reactivity with CDC27 homologues from other sources has not been determined.
Target Information
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AN CDC27Hs; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; CDC27 homolog; CDC27Hs; cell division cycle 27 homolog; Cell division cycle protein 27 homolog; D0S1430E, D17S978E; H-NUC; nuc2 homolog; nuclear DNA binding protein; unnamed protein product
Gene Aliases: ANAPC3; APC3; CDC27; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2
UniProt ID: (Human) G3V1C4
Entrez Gene ID: (Human) 996
metaphase/anaphase transition of mitotic cell cycle
regulation of mitotic cell cycle
protein ubiquitination
anaphase-promoting complex-dependent catabolic process
neuron projection development
cell division
regulation of meiotic cell cycle
protein K48-linked ubiquitination
protein K11-linked ubiquitination
protein branched polyubiquitination
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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