SuperSignal™ Molecular Weight Protein Ladder

El patrón proteico de peso molecular Thermo Scientific SuperSignal (de 20 a 150 kDa) es una mezcla de ocho proteínasMás información

Número de catálogo	Cantidad
84785	250 μl

Número de catálogo 84785

Precio (MXN)

Cantidad:

250 μl

Pedido a granel o personalizado

El patrón proteico de peso molecular Thermo Scientific SuperSignal (de 20 a 150 kDa) es una mezcla de ocho proteínas recombinantes sin teñir con lugares de unión de IgG para sistemas quimioluminiscentes, fluorescentes, cromogénicos u otros sistemas de detección. El patrón proteico se suministra en un formato listo para usar para la carga directa en geles; sin necesidad de calentar, reducir ni añadir un tampón de muestras antes de usarlo.

Compare y visualice todos los demás patrones y escaleras de proteínas ›

Las proteínas recombinantes del patrón se unen a los anticuerpos utilizados en la inmunotransferencia a través del sitio de unión de IgG. Los marcadores de proteínas se pueden visualizar utilizando sustratos adecuados para anticuerpos etiquetados con enzimas o a través de anticuerpos fluorescentes etiquetados con colorantes (no recomendados para anticuerpos etiquetados con fluoruros en el canal 500–550 nm).

Se ofrecen dos versiones del marcador MW. La fórmula es adecuada para utilizarse con la mayoría de los anticuerpos policlonales de conejo y con otros anticuerpos policlonales que no sean de ratón. La fórmula del patrón proteico de peso molecular mejorado SuperSignal se ha concebido en especial para aplicaciones que requieran anticuerpos monoclonales de ratón y experimentos en los que se utilicen concentraciones muy diluidas de anticuerpos. Por ejemplo, el patrón mejorado puede ser necesario cuando se utiliza una dilución de 1:50.000 (25 ng/ml) a 1:100.000 (10 ng/ml) del anticuerpo secundario.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Método de detecciónSistema de detección definido por el usuario

Compatibilidad del gelGeles Bolt™ Bis-Tris Plus, Geles de tricina Novex™, geles de Tris-glicina Novex™, geles NuPAGE™ Bis-Tris, geles de Tris-acetato NuPAGE™, geles SDS-PAGE

Peso molecular150, 100, 80, 60, 50, 40, 30, 20 kDa

Línea de productosSuperSignal

Tipo de productoMarcadores moleculares de proteínas

Cantidad250 μl

Listo para cargarSí

Condiciones de envíoHielo húmedo

Tipo de tinciónSin teñir

Tipo de sistemaWestern Blotting, SDS-PAGE

Number of Markers8

Intervalo de tamañosDe 20 a 150 kDa

Unit SizeEach

Contenido y almacenamiento

Contenido: un vial de 250 μl

Tampón de almacenamiento: 62,5 mm de Tris-H₃PO₄ (pH de 7,5 a 25 °C), 1 mm de EDTA, SDS al 2 %, 10 mm de DTT, 1 mm de NaN₃ y glicerol al 33 %

Almacenamiento: Al recibirlo, almacénelo en -20°C para el almacenamiento a largo plazo, o tres meses en 4°C o un mes en el ambiente

Preguntas frecuentes

With your SuperSignal Molecular Weight Protein Ladder/ SuperSignal Enhanced MW Protein Ladder, I see dye front interference in fluorescent applications. What should I do?

This is likely due to the dye-front not run off the gel or removed from the blot. We recommend running the dye front off of the gel or removing it from the blot.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used your SuperSignal Molecular Weight Protein Ladder and did not see any bands after western detection. What could have happened?

Here are possible causes and solutions:

- Not enough volume loaded: Load more volume onto the gel
- Incomplete or poor transfer: Optimize transfer conditions
- Secondary antibody does not recognize marker proteins: Ensure appropriate secondary is used that binds to marker proteins
- Secondary antibody not enzyme-conjugated: Ensure appropriate secondary antibody used in system
- A mouse monoclonal primary antibody was used: Use a greater volume of the ladder or use SuperSignal Enhanced Molecular Weight Protein Ladder (Cat. No. 84786) for mouse primary antibodies

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With your SuperSignal Molecular Weight Protein Ladder and SuperSignal Enhanced MW Protein Ladder, I did not get visible band separation after western detection. What could have happened?

Here are possible causes and solutions:

- Ladder was boiled: Discard boiled aliquot.
- Too much volume of ladder used: Load less volume of the ladder.
- Concentration of antibody is too high: Optimize antibody concentration

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used one of your protein standards for a western transfer and noticed that some of the lower-molecular weight protein bands passed through the membrane. How can I resolve this issue?

- Decrease voltage, current or length of transfer time
- Make sure that the methanol concentration in the transfer buffer is proper; use a methanol concentration of 10-20% methanol removes the SDS from SDS-protein complexes and improves the binding of protein to the membrane.
- Make sure that the SDS concentration (if added) in the transfer buffer is proper, don't use more than 0.02-0.04% SDS. Using too much SDS can prevent binding of proteins to the membrane.
- Check the pore size of the membrane and the size of the target protein. Proteins smaller than 10 kDa will easily pass through a 0.45 µm pore size membrane. If proteins smaller than 10 kDa are of interest, it would be better to use a 0.2 µm pore size membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used one of your protein standards for a western transfer and noticed that some of the higher-molecular weight bands transferred very poorly to the membrane. Can you offer some tips?

- Increase voltage, current or length of time for transfer
- SDS in the gel and in the SDS-protein complexes promotes elution of the protein from the gels but inhibits binding of the protein to membranes. This inhibition is higher for nitrocellulose than for PVDF. For proteins that are difficult to elute from the gel such as large molecular weight proteins, a small amount of SDS may be added to the transfer buffer to improve transfer. We recommend pre-equilibrating the gel in 2X Transfer buffer (without methanol) containing 0.02-0.04% SDS for 10 minutes before assembling the sandwich and then transferring using 1X transfer buffer containing 10% methanol and 0.01%SDS.
- Methanol removes the SDS from SDS-protein complexes and improves the binding of protein to the membrane, but has some negative effects on the gel itself, leading to a decrease in transfer efficiency. It may cause a reduction in pore size, precipitation of some proteins, and some basic proteins to become positively charged or neutral. Make sure that the methanol concentration in the transfer buffer is not more than 10-20% and that high-quality, analytical grade methanol is used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all SuperSignal™ Molecular Weight Protein Ladder FAQs