A-23187 Free Acid (Calcimycin)
A-23187 Free Acid (Calcimycin)
Citas y referencias (397)
Invitrogen™

A-23187 Free Acid (Calcimycin)

El ionóforo Ca2+ A-23187 se utiliza comúnmente para calibraciones in situ de indicadores fluorescentes de Ca2+, para equilibrar las concentracionesMás información
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Número de catálogoCantidad
A149310 mg
Número de catálogo A1493
Precio (MXN)
-
Cantidad:
10 mg
El ionóforo Ca2+ A-23187 se utiliza comúnmente para calibraciones in situ de indicadores fluorescentes de Ca2+, para equilibrar las concentraciones intracelulares y extracelulares de Ca2+ y para permitir que Mn2+ entre en la célula para apagar la fluorescencia de colorante intracelular. Aunque la fluorescencia intrínseca de A-23197 es demasiado alta para su uso con indicadores de calcio excitables con UV como fura-2, indo-1 y quin-2, es adecuada para su uso con indicadores excitables con luz visible, incluidos fluo-4, fluo-3, Calcium Green y rhod-2. A-23187 también se puede utilizar para equilibrar concentraciones extracelulares e intracelulares de Mg2+, lo que lo hace útil para calibrar indicadores de Mg2+.

Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de la membrana ›

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia, citómetro de flujo, fluorímetro, imagen fluorescente
IndicadorIndicador de calcio
Fórmula molecularC29H37N3O6
Cantidad10 mg
Almacenamiento recomendadoAlmacenar en el congelador (de -5 °C a -30 °C) y proteger de la luz.
Condiciones de envíoTemperatura ambiente
Forma físicaSólido
Tipo de productoCalcimicina
Unit SizeEach

Citations & References (397)

Citations & References
Abstract
Flow cytometric measurement of the production of reactive oxygen intermediate in activated rat mast cells.
Authors:Tsinkalovsky OR, Laerum OD
Journal:APMIS
PubMed ID:7520710
The production of reactive oxygen intermediates (ROI) in compound 48/80- or calcium ionophore A 23187- activated pleural or peritoneal mast cells was monitored using flow cytometry and the fluorescence indicator dihydrorhodamine 123 (a derivate of rhodamine 123). Mast cell degranulation and ROI production were estimated by flow cytometric fluorescence and ... More
Inhibition of NADPH oxidase activation by 4-(2-aminoethyl)-benzenesulfonyl fluoride and related compounds.
Authors:Diatchuk V, Lotan O, Koshkin V, Wikstroem P, Pick E
Journal:J Biol Chem
PubMed ID:9148950
The elicitation of an oxidative burst in phagocytes rests on the assembly of a multicomponental complex (NADPH oxidase) consisting of a membrane-associated flavocytochrome (cytochrome b559), representing the redox element responsible for the NADPH-dependent reduction of oxygen to superoxide (O-2), two cytosolic components (p47(phox), p67(phox)), and the small GTPase Rac (1 ... More
Exocytotic insertion of calcium channels constrains compensatory endocytosis to sites of exocytosis.
Authors:Smith RM, Baibakov B, Ikebuchi Y, White BH, Lambert NA, Kaczmarek LK, Vogel SS
Journal:J Cell Biol
PubMed ID:10684256
'Proteins inserted into the cell surface by exocytosis are thought to be retrieved by compensatory endocytosis, suggesting that retrieval requires granule proteins. In sea urchin eggs, calcium influx through P-type calcium channels is required for retrieval, and the large size of sea urchin secretory granules permits the direct observation of ... More
Involvement of nuclear factor of activated T cells activation in UV response. Evidence from cell culture and transgenic mice.
Authors:Huang C, Mattjus P, Ma WY, Rincon M, Chen NY, Brown RE, Dong Z
Journal:J Biol Chem
PubMed ID:10734048
'Mammalian cells respond to UV radiation by signaling cascades leading to activation of transcription factors, such as activated protein 1, NFkappaB, and p53, a process known as the "UV response." Nuclear factor of activated T cells (NFAT) was first identified as an inducible nuclear factor in immune response and subsequently ... More
Potentiation of CD3-induced expression of the linker for activation of T cells (LAT) by the calcineurin inhibitors cyclosporin A and FK506.
Authors:Peters D, Tsuchida M, Manthei ER, Alam T, Cho CS, Knechtle SJ, Hamawy MM
Journal:Blood
PubMed ID:10779414
'The activation of blood cells, including T cells, triggers intracellular signals that control the expression of critical molecules, including cytokines and cytokine receptors. We show that T-cell receptor (TCR) ligation increases the cellular level of the protein linker for activation of T cells (LAT), a molecule critical for T-cell development ... More
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