E-Gel™ NGS™ 0.8% Agarose Gels
E-Gel™ NGS™ 0.8% Agarose Gels
Invitrogen™

E-Gel™ NGS™ 0.8% Agarose Gels

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Los geles de agarosa E-Gel NGS al 0,8 % son geles de agarosa autónomos y prefundidos diseñados para proporcionar unaMás información
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Número de catálogoCantidad
A2579810 geles
Número de catálogo A25798
Precio (MXN)
-
Cantidad:
10 geles
Los geles de agarosa E-Gel NGS al 0,8 % son geles de agarosa autónomos y prefundidos diseñados para proporcionar una electroforesis rápida, cómoda y sencilla. Cada gel de agarosa E-Gel está listo para su uso con agarosa, electrodos y tinción de gel de ADN SYBR Safe envasados dentro de un casete transparente de 11 pocillos, fácil de abrir, con luz UV y azul. La tinción SYBR Safe se puede visualizar con luz azul, lo que minimiza el daño al ADN y permite una supervisión segura y en tiempo real de la migración del ADN mediante el sistema de electroforesis E-Gel Power Snap.

Fácil acceso al gel
Para abrir el casete de gel se debe utilizar la espátula para geles, lo que permite acceder fácilmente al gel para la escisión de bandas específicas o transferirlo a una membrana para el análisis de Southern blot.

Marcadores de ácido nucleico
Obtenga más información sobre nuestras escaleras de DNA E-Gel ›

Aparatos para la ejecución y la visualización
Los geles E-Gel NGS requieren el sistema de electroforesis E-Gel Power Snap para ejecutar y visualizar el gel en tiempo real.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Compatible LaddersMarcador de ADN E-Gel™ Plus de 1 kb
Características ecológicasMenos peligroso
Tipo de productoGel de agarosa
Cantidad10 geles
Tipo de tinciónSYBR™ Safe
Diseño del pocilloPeine simple
Porcentaje del gel0,8%
Tipo de gelE-Gel
Intervalo de separaciónDe 800 bp a 10 kb
Pocillos11 pocillo
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente.

Preguntas frecuentes

How can I view DNA on my E-Gel agarose gel with SYBR Safe DNA stain?

SYBR Safe-stained gels can be visualized with a standard UV transilluminator, a laser-based scanner equipped with an excitation source in the UV range or between 470 and 530 nm, or a blue-light transilluminator.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

My E-Gel agarose gel has speckles when viewed in the imager. What should I do?

Here are some suggestions:

- Try cleaning the cassettes with alcohol and Kimwipes wipers.
- Try cleaning the camera lens.
- Try to adjust the exposure time and brightness options of the documentation system you are using.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

My sample is leaking from the wells when running my E-Gel agarose gels. What happened?

Please ensure that you have not overloaded the well and that the wells were not damaged during comb removal.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

I accidentally stored my E-Gel agarose gels at 4 degrees C instead of room temperature. Can I still use them?

While we recommend storage at room temperature, these gels will still be usable. Bring the gels to room temperature prior to the run for optimal conditions.

Find additional tips, troubleshooting help, and resources within ourNucleic Acid Gel Electrophoresis and Blotting Support Center.

What loading buffer should I use for my E-Gel agarose gels?

Loading buffer is optional. Samples can be loaded directly into the wells if no buffer is used, or you can dilute them with deionized water or TE buffer. If you want to use a loading buffer, please see the recipes below:

E-Gel agarose gels (including EX)
10 mM Tris-HCl, pH 7.5
1 mM EDTA
0.005% bromophenol blue
0.005% xylene cyanol FF
E-Gel CloneWell II and E-Gel SizeSelect II agarose gels
10 mM Tris-HCl, pH 7.5
1 mM EDTA

Alternatively, you can use 10X BlueJuice Gel Loading Buffer or TrackIt Loading Buffer. Dilute this buffer 50- to 200-fold to obtain optimal results with E-Gel agarose gels.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

View all E-Gel™ NGS™ 0.8% Agarose Gels FAQs