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Citas y referencias (14)
Invitrogen™
5-CFDA, AM (5-Carboxyfluorescein Diacetato, acetoximetil Ester)
5-CFDA, AM es un sustrato de esterasa que penetra en las células y que puede servir como sonda de viabilidadMás información
| Número de catálogo | Cantidad |
|---|---|
| C1354 | 5 mg |
Número de catálogo C1354
Precio (MXN)
-
Cantidad:
5 mg
5-CFDA, AM es un sustrato de esterasa que penetra en las células y que puede servir como sonda de viabilidad que mide tanto la actividad enzimática, que es necesaria para activar su fluorescencia, como la integridad célula-membrana, que es necesaria para la retención intracelular de su producto fluorescente. El éster AM electrónicamente neutro de CFDA puede ser cargado en células en concentraciones más bajas que la CFDA. Tras la hidrólisis por esterasas intracelulares, este éster AM también produce carboxifluoresceína. En comparación con la fluoresceína, la carboxifluoresceína contiene cargas negativas adicionales y, por lo tanto, se conserva mejor en las células.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de coloranteOtras etiquetas o colorantes
Cantidad5 mg
Tipo de reactivoCFDA y compuestos relacionados
Condiciones de envíoTemperatura ambiente
Tipo de productoAM 5-CFDA
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador entre -5 °C y -30 °C
Citations & References (14)
Citations & References
Abstract
Calcein: a novel marker for lymphocytes which enter lymph nodes.
Journal:Cytometry
PubMed ID:1451604
Previous studies have identified unique cell surface antigens which are associated with the specific binding of lymphocytes to high endothelial venules (HEV). Evidence is presented in this paper which demonstrates that uptake of the fluorescent dye calcein by lymphocytes represents an additional marker for the lymph node homing subpopulation of
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent
Ability of 16 priority PAHs to be photocytotoxic to a cell line from the rainbow trout gill.
Journal:Toxicology
PubMed ID:9699801
'Sixteen polycyclic aromatic hydrocarbons (PAHs) were screened for their ability to be photocytotoxic to a cell line from the rainbow trout gill, RTgill-W1. PAHs could be divided into one of three groups: incapable of being photocytotoxic, able to be both photocytotoxic and directly cytotoxic, or capable of being only photocytotoxic.
Cell death-associated translocation of plasma membrane components induced by CTL.
Journal:J Immunol
PubMed ID:10779768
'In the very early stages of target cell apoptosis induced by CTL, we found that fluorescence of labeling probes of the target plasma membrane, such as N-(3-triethylammoniumpropyl)-4-(p-dibutylaminostyryl)pyridin ium dibromide (FM1-43), was translocated into intracellular membrane structures including nuclear envelope and mitochondria. This translocation was associated with the execution of CTL-mediated
Comparison of four different colorimetric and fluorometric cytotoxicity assays in a zebrafish liver cell line.
Journal:BMC Pharmacol
PubMed ID:18513395
'BACKGROUND: A broad spectrum of cytotoxicity assays is currently used in the fields of (eco)toxicology and pharmacology. To choose an appropriate assay, different parameters like test compounds, detection mechanism, specificity, and sensitivity have to be considered. Furthermore, tissue or cell line can influence test performance. For zebrafish (Danio rerio), as