El kit de tampón SDS de tris-acetato NuPAGE™ (para geles de tris-acetato), contiene 1 unidad. LA0041, NP0004, NP0005, NP0007
El kit de tampón SDS de tris-acetato NuPAGE&trade; (para geles de tris-acetato), <i>contiene 1 unidad. LA0041, NP0004, NP0005, NP0007</i>
El kit de tampón SDS de tris-acetato NuPAGE&trade; (para geles de tris-acetato), <i>contiene 1 unidad. LA0041, NP0004, NP0005, NP0007</i>
El kit de tampón SDS de tris-acetato NuPAGE&trade; (para geles de tris-acetato), <i>contiene 1 unidad. LA0041, NP0004, NP0005, NP0007</i>
El kit de tampón SDS de tris-acetato NuPAGE&trade; (para geles de tris-acetato), <i>contiene 1 unidad. LA0041, NP0004, NP0005, NP0007</i>
El kit de tampón SDS de tris-acetato NuPAGE&trade; (para geles de tris-acetato), <i>contiene 1 unidad. LA0041, NP0004, NP0005, NP0007</i>
Invitrogen™

El kit de tampón SDS de tris-acetato NuPAGE™ (para geles de tris-acetato), contiene 1 unidad. LA0041, NP0004, NP0005, NP0007

El kit de tampón de SDS de Tris-acetato NuPAGE está diseñado para la separación de proteínas de tamaño medio aMás información
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Número de catálogoCantidad
LA00501 kit
Número de catálogo LA0050
Precio (MXN)
-
Cantidad:
1 kit
El kit de tampón de SDS de Tris-acetato NuPAGE está diseñado para la separación de proteínas de tamaño medio a grande en geles de Tris-acetato. Este kit incluye los siguientes tampones:
• Tampón de deslizamiento de SDS de Tris-acetato NuPAGE (20X, n.º de cat. LA0041)
• Agente reductor de muestras NuPAGE (10X, 250 µl, n.º de cat. NP0004)
• Antioxidante NuPAGE (n.º de cat. NP0005)
• Tampón de muestra de LDS NuPAGE (4X, 10 ml, n.º de cat. NP0007)

Consulte todos los tampones y reactivos disponibles para SDS-PAGE

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de gelGel SDS PAGE
Cantidad1 kit
Gel CompatibilityGel de Tris-acetato NuPAGE™
Línea de productosNuPAGE
Tipo de productoKit del tampón
Unit SizeEach
Contenido y almacenamiento
El kit de tampón de SDS de Tris-acetato NuPAGE™ (para geles de Tris-acetato) contiene:

• Tampón de desplazamiento de SDS de Tris-acetato NuPAGE™ 20X (500 ml): Almacenar de 4 °C a 25 °C

• Agente reductor de muestras 10X NuPAGE™ (250 µl): Almacenar de 2 °C a 8 °C

• Antioxidante NuPAGE™ (15 ml): Almacenar a 4 °C

• Tampón de muestra de LDS 4X NuPAGE™ (10 ml): Almacenar entre 4 °C y 25 °C

Preguntas frecuentes

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can I use CTAB rather than SDS in my sample buffer?

No, CTAB will not work with any of our gels except for the NuPAGE Tris-Acetate gels. To use CTAB, you would need to use a running buffer of 50 mM acetic acid and 50 mM beta-alanine in equal concentrations. You would also need to switch the electrodes. Since CTAB is a cationic detergent, this would establish conditions for running a basic protein towards the anode (into the gel).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is there a reasonable replacement for the NuPAGE antioxidant for gel electrophoresis?

One can use 0.002% thioglycolic acid in the upper buffer reservoir. This is a good scavenger of free radicals. The reference to this is described by Hunkapiller et al, Methods of Enzymology, (91), 399, 1983. Caution should be taken when using this method since this compound is both toxic and expensive. In addition, the TGA must be fresh as it tends to become oxidized itself over time. Oxidized TGA will actually promote sample re-oxidation.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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