SimplyBlue™ SafeStain

Citas y referencias (5)

Invitrogen™

SimplyBlue™ SafeStain

Name: SimplyBlue™ SafeStain
SKU: LC6060

SimplyBlue SafeStain es una tinción Coomassie G-250 lista para usar, rápida, sensible y segura para visualizar las bandas de proteínasMás información

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Número de catálogo	Cantidad
LC6060	1 l
LC6065	3,5 L

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Número de catálogo LC6060

Precio (MXN)

Cantidad:

1 l

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SimplyBlue SafeStain es una tinción Coomassie G-250 lista para usar, rápida, sensible y segura para visualizar las bandas de proteínas en geles de poliacrilamida y en membranas de PVDF secas. Es totalmente inocua y no requiere decolorantes ni fijadores de metanol ni ácido acético. El riesgo a una exposición peligrosa y los olores desagradables se han eliminado y se crea un entorno más saludable en el laboratorio.

Compare todas las tinciones de Coomassie ›

Protocolo fácil de usar
El protocolo de SimplyBlue SafeStain es fácil de seguir y puede finalizarse en menos de tres horas. Para mayor velocidad, un simple procedimiento de microondas se puede finalizar en 12 minutos. La decoloración no es necesaria, pero puede realizarse con agua desionizada para lograr la máxima sensibilidad, especialmente al realizar análisis posteriores, como la espectrometría de masas o cuando se requiere un fondo transparente.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Ubicación de detecciónDetección en gel

Método de detecciónColorimétrico

Características ecológicasMenos peligroso

Línea de productosSimplyBlue

Tipo de productoTinción segura de proteínas

Cantidad1 l

Duración de almacenamiento6 meses

Molécula dianaproteína

ColorBlue

Etiqueta o tinteCoomassie

Unit SizeEach

Contenido y almacenamiento

SimplyBlue™ SafeStain se suministra como reactivo de tinción listo para usar 1X. Almacenar a temperatura ambiente. Se garantiza la estabilidad durante 6 meses si se almacena correctamente. No hay cargos de HazMat asociados con este producto.

Preguntas frecuentes

Can I use the Colloidal Blue Staining Kit (Cat. No. LC6025) for membrane staining to detect proteins that are transferred to a membrane?

It is not recommended because the background will be too high. Better alternatives include: 1) Invitrogen Reversible Membrane Protein Stain Kit (Cat. No. IB7710).

2) Coomassie (non-colloidal) staining: stain in 0.1% Coomassie Blue R-250 in 50% methanol for 5 min and destain with several changes of 50% methanol and 10% acetic acid. Rinse with several changes of water, air dry and store for up to 12 months at -20°C. Sensitivity is approximately at the 50-100 ng level.

3) Use SimplyBlue SafeStain (Cat. No. LC6060). The SimplyBlue SafeStain manual has the protocol for staining PVDF membranes, but it is not recommended for nitrocellulose because of high background.

4) Amido Black: same as Coomassie but less sensitive.

5) Ponceau S: same as Coomassie but less sensitive.

6) UV transillumination: place membrane on filter paper after blot is finished and allow to dry at room temperature for about 10 min. Rewet in 20% methanol and view the blot in front of white light while it is still wet; the bands will look more translucent than the membrane. If the bands disappear as the membranes dries, rewet again.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why is Coomassie G-250 used as the tracking dye in NuPAGE LDS Sample Buffer instead of bromophenol blue?

Coomassie G-250 will give a sharp dye front with both NuPAGE MES and MOPS Running Buffers and is therefore used as the tracking dye in the NuPAGE LDS Sample Buffer.

Bromophenol blue runs more slowly than some peptides with the NuPAGE MES Running Buffer system.

Coomassie G-250 migrates much closer to the moving ion front than bromophenol blue, ensuring that small peptides will not be run too far (e.g., off the gel).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the SimplyBlue SafeStain the appropriate stain for quantitation by densitometry?

The Colloidal Blue Staining Kit (Cat. No. LC6025) is best for quantitation by densitometry. You can also use SimplyBlue SafeStain for this application.

The great advantage of SimplyBlue SafeStain is that it is very easy to use and safe.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The sensitivity of my SimplyBlue SafeStain seems to be decreasing over time. Why is this?

Check the cap on the bottle. If the bottles are not tightly sealed, the alcohol can evaporate from the stain causing substantial decrease in stain sensitivity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How do I destain proteins on a PVDF membrane that were stained with SimplyBlue SafeStain?

After staining with SimplyBlue SafeStain, use deionized water for the less strongly retained protein bands on the PVDF membrane.

Increasing methanol or ethanol concentrations up to 70% should destain any remaining bands. You can leave the membrane in the destain indefinitely.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all SimplyBlue™ SafeStain FAQs

Citations & References (5)

Citations & References

Abstract

Enzymatic hydrolysis of pyridoxine-5'-beta-D-glucoside is catalyzed by intestinal lactase-phlorizin hydrolase.

Authors: Mackey Amy D; Henderson George N; Gregory Jesse F 3rd;

Journal:J Biol Chem

PubMed ID:12023280

'An obligatory step in the mammalian nutritional utilization of pyridoxine-5''-beta-D-glucoside (PNG) is the intestinal hydrolysis of its beta-glucosidic bond that releases pyridoxine (PN). This laboratory previously reported the purification and partial characterization of a novel cytosolic enzyme, designated PNG hydrolase, which hydrolyzed PNG. An investigation of the subcellular distribution of ... More

2F3 monoclonal antibody recognizes the O26 O-antigen moiety of the lipopolysaccharide of enterohemorrhagic Escherichia coli strain 4276.

Authors:Szalo IM, Taminiau B, Goffaux F, Pirson V, McCappin J, Ball HJ, Mainil JG,

Journal:Clin Diagn Lab Immunol

PubMed ID:15138178

'Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) organisms are groups of pathogenic strains whose infections are characterized by a typical lesion of enterocyte attachment and effacement. They are involved in enteric diseases both in humans and in animals, and EHEC strains can be responsible for hemolytic uremic syndrome ... More

Selective fluorescent labeling of S-nitrosothiols (S-FLOS): a novel method for studying S-nitrosation.

Authors:Santhanam L, Gucek M, Brown TR, Mansharamani M, Ryoo S, Lemmon CA, Romer L, Shoukas AA, Berkowitz DE, Cole RN,

Journal:Nitric Oxide

PubMed ID:18706513

'Protein S-nitrosation is a reversible post-translation modification critical for redox-sensitive cell signaling that is typically studied using the Biotin Switch method. This method and subsequent modifications usually require avidin binding or Western blot analysis to detect biotin labeled proteins. We describe here a modification of the Biotin Switch assay that ... More

Vascular endothelial growth factor-C and C-C chemokine receptor 7 in tumor cell-lymphatic cross-talk promote invasive phenotype.

Authors:Issa A, Le TX, Shoushtari AN, Shields JD, Swartz MA,

Journal:Cancer Res

PubMed ID:19118020

'Most carcinomas spread to distant sites through lymphatic vessels. Several preclinical and clinical studies have shown a positive correlation between the incidence of lymph node metastasis and secretion of the lymphatic growth factor vascular endothelial growth factor-C (VEGF-C) by tumor cells, suggesting tumor lymphangiogenesis as an escape mechanism. However, recent ... More

Yeast Expression and NMR Analysis of the Extracellular Domain of Muscle Nicotinic Acetylcholine Receptor alpha Subunit.

Authors: Yao Yun; Wang Junmei; Viroonchatapan Nitnara; Samson Avraham; Chill Jordan; Rothe Elizabeth; Anglister Jacob; Wang Zuo-Zhong;

Journal:J Biol Chem

PubMed ID:11812776

The alpha subunit of the nicotinic acetylcholine receptor (AChR) from Torpedo electric organ and mammalian muscle contains high affinity binding sites for alpha-bungarotoxin and for autoimmune antibodies in sera of patients with myasthenia gravis. To obtain sufficient materials for structural studies of the receptor-ligand complexes, we have expressed part of ... More