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Citas y referencias (86)
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Mag-Fura-2, Tetrapotassium Salt, cell impermeant
Mag-fura-2, sal de tetrapotasio es un indicador de magnesio intracelular de alta afinidad que es radiométrico y excitable mediante luzMás información
| Número de catálogo | Cantidad |
|---|---|
| M1290 | 1 mg |
Número de catálogo M1290
Precio (MXN)
-
Cantidad:
1 mg
Mag-fura-2, sal de tetrapotasio es un indicador de magnesio intracelular de alta afinidad que es radiométrico y excitable mediante luz UV. Esta forma de sal soluble en agua es útil para la carga intracelular mediante microinyección, infusión con una pipeta de parche o captación inducida mediante nuestro reactivo de carga celular pinocítica Influx (I14402).
Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de membrana ›
Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de membrana ›
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteA base de colorantes fluorescentes
Cantidad1 mg
Condiciones de envíoTemperatura ambiente
Para utilizar con (equipo)Microscopio de fluorescencia, Microfotómetro, Citómetro de flujo
Tipo de productoIndicador de magnesio
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (86)
Citations & References
Abstract
Ca2+ transients in cardiac myocytes measured with high and low affinity Ca2+ indicators.
Journal:Biophysical journal
PubMed ID:8274651
Intracellular calcium ion ([Ca2+]i) transients were measured in voltage-clamped rat cardiac myocytes with fura-2 or furaptra to quantitate rapid changes in [Ca2+]i. Patch electrode solutions contained the K+ salt of fura-2 (50 microM) or furaptra (300 microM). With identical experimental conditions, peak amplitude of stimulated [Ca2+]i transients in furaptra-loaded myocytes
Matrix free Mg2+ changes with metabolic state in isolated heart mitochondria.
Journal:Biochemistry
PubMed ID:2361136
'The concentration of free Mg2+ in the matrix of isolated heart mitochondria has been monitored by using the fluorescent probe furaptra (mag-fura-2). Beef heart mitochondria respiring in a KCl medium in the absence of external Mg2+ maintain free matrix Mg2+ near 0.50 mM. Addition of Pi under these conditions decreases
Modulation of Mg2+ efflux from rat ventricular myocytes studied with the fluorescent indicator furaptra.
Journal:Biophys J
PubMed ID:15626700
'The fluorescent Mg(2+) indicator furaptra (mag-fura-2) was introduced into single ventricular myocytes by incubation with its acetoxy-methyl ester form. The ratio of furaptra's fluorescence intensity at 382 and 350 nm was used to estimate the apparent cytoplasmic [Mg(2+)] ([Mg(2+)](i)). In Ca(2+)-free extracellular conditions (0.1 mM EGTA) at 25 degrees C,
Mg2+ buffering in cultured chick ventricular myocytes: quantitation and modulation by Ca2+.
Journal:Am J Physiol
PubMed ID:8498485
'To characterize the Mg2+ buffering of cultured chick ventricular myocytes, cytosolic Mg2+ was increased by liberating Mg2+ normally chelated by ATP upon total depletion of ATP content. Because the total Mg content and cell volume remained constant during this time, the difference between the amount of Mg2+ liberated (2.7 mM)
Capacitative Ca2+ entry is closely linked to the filling state of internal Ca2+ stores: a study using simultaneous measurements of ICRAC and intraluminal [Ca2+].
Journal:J Cell Biol
PubMed ID:9442108
'ICRAC (the best characterized Ca2+ current activated by store depletion) was monitored concurrently for the first time with [Ca2+] changes in internal stores. To establish the quantitative and kinetic relationship between these two parameters, we have developed a novel means to clamp [Ca2+] within stores of intact cells at any